Cell-Free Synthesis of Defined N-Glycoconjugates

Cell-free Glycosylation

Unlocking Complex Glycoproteins: A Co-Translational Mechanism in Cell-Free Systems
October 24, 2024
Unlocking Complex Glycoproteins: A Co-Translational Mechanism in Cell-Free Systems

On July 8, 2025, Bidstrup et al., representing institutions such as Cornell University, the University of Maryland, and Stanford University, published a paper entitled "Glycosylation of Structured Protein Domains in Cell-Free Reaction Environments" in ACS Synthetic Biology. The article addresses a fundamental limitation in biomanufacturing: the challenge of predictably attaching N-linked glycans to protein targets […]

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Cell-Free Synthesis of Defined <em>N</em>-Glycoconjugates
December 4, 2025
Cell-Free Synthesis of Defined N-Glycoconjugates

On Sep 12, 2023, Wenzel et al. from the Max Planck Institute for Dynamics of Complex Technical Systems, published a paper in the Frontiers in Molecular Biosciences, titled " Cell-free N-glycosylation of peptides using synthetic lipid-linked hybrid and complex N-glycans". The research addresses one of the most persistent challenges in biotechnology: the production of proteins […]

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