Chinese Hamster Ovary (CHO) Engineering Service
Client Satisfaction with Chinese Hamster Ovary (CHO) Engineering Service Is Our Motivation
CD BioGlyco has extensive experience in developing Mammalian Chassis for Synthetic Biology (CHO cells, HEK293 Cells). CHO cells have been adapted for the Production of glycosaminoglycans, glycoproteins, and other substances. It is one of the ideal engineering Chassis. We provide a one-stop CHO glycoengineering service to help clients accelerate the production of glycoproteins and others, functional resolution, and other research. Based on our extensive experience in Chassis Development, we have a precise and scalable set of tools to characterize and control gene expression and function in CHO cells, culminating in stable CHO cell lines.
- CHO glycoengineering
CHO cells dominate the production of glycoproteins, antibodies, etc. We design, construct, and characterize synthetic genetic pathways that ultimately enable CHO cells to produce a wide variety of glycoproteins, glycans, antibodies, and other assorted products predictably and stably.
Design: We combine computational system analysis and bioinformatics tools to rationalize the design of synthetic pathways.
Modification: We have various genome editing tools to precisely edit the CHO genome.
Validation of CHO mutants.
- Genome editing tools
Site-specific recombination: This method relies on fungal or bacterial recombinases.
Clustered regularly interspaced short palindromic repeats (CRISPR) system: This method relies on DNA endonucleases, which are flexible enough to target DNA through short RNA rather than protein structural domains. It also mutates multiple alleles at the same time.
Transcription activator-like effector nucleases (TALEN) gene editing: This method is used to cut DNA at a specific location for gene editing.
Multiplexed genome engineering (MAGE): This method relies on oligonucleotide-mediated recombination and is a method for editing multiple genetic loci in parallel.
- What do we deliver?
Construction of CHO cell line
Carbohydrate-related products
Cell line validation results
Experimental records and reports
Fig.1 Flowchart of our CHO glycoengineering. (CD BioGlyco)
Publication Data
Technology: Gene editing
Journal: Frontiers in Bioengineering and Biotechnology
IF: 5.7
Published: 2023
Results: In this study, a CHO cell was designed and constructed for the expression of antibodies initially produced in a murine cell line to produce murine glycans. Validated results showed that the two CHO cells constructed maintained stable production after 65 population doublings, while the glycosylation profile and function of the product matched the control. This study provides an effective strategy for engineering CHO glycans for glycan production.
Fig.2 Methods of chassis cell construction. (Gupta, et al., 2023)
Frequently Asked Questions
- What does the client need to provide when carrying out CHO glycoengineering?
- Gene and vector information to be cloned
- Cell lines to be transfected
- Positive control samples
- Relevant literature (optional)
- Others (to be provided according to specific experimental design)
- What are the advantages of CHO cells for synthetic biology?
As the mainstay of the current synthetic biology field for the production of recombinant proteins and the like, CHO cells have many advantages. It is fast-growing, easily transfected adapted to serum-free suspension culture, safe, and has a mature production process. In addition, its expression system is characterized by high expression, higher density culture, and a relatively complete protein glycosylation modification system, making it one of the ideal chassis cells.
Applications
- CHO glycoengineering is used for the production of biotherapeutic glycoproteins.
- Expanding the possibilities of regulating biological systems through CHO glycoengineering opens up the possibility of developing new therapeutic interventions.
- CHO glycoengineering is used for the continuous and stable production of various types of glycans.
Advantages of Us
- We have a variety of technologies for CHO glycoengineering to address the challenges of delivering large amounts of exogenous DNA into cells.
- We integrate target genes at genome-specific sites through site-specific integration to avoid the instability associated with random integration.
- We provide a full range of services from information collection to feasibility analysis, and from program design to service delivery.
CD BioGlyco has extensive experience in gene editing and cellular modification. We offer a variety of chassis cell constructs for synthetic biology. Please feel free to contact us for more information about CHO glycoengineering. Our well-established project management system will ensure the timely delivery of CHO cells.
Reference
- Gupta, S.; et al. Engineering protein glycosylation in CHO cells to be highly similar to murine host cells. Frontiers in Bioengineering and Biotechnology. 2023, 11: 182.
For research use only. Not intended for any clinical use.
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