We Provide the Custom 3-Fucosyllactose-based Cell-free System for Clients

CD BioGlyco is well-equipped to provide the 3-fucosyllactose-based cell-free system construction service. Our expertise in cell-free protein synthesis (CFPS) systems allows us to express the necessary glycosyltransferases, including fucosyltransferases, in vitro. Besides, we also provide a powerful Cell-based Production System based on our synthetic biology platform. By leveraging our advanced technology and experience in glycobiology, we offer a comprehensive service for constructing cell-free systems tailored to the synthesis of complex glycan structures like 3-fucosyllactose. Researchers can rely on our expertise to develop and optimize protocols for the precise production of desired glycans in a controlled laboratory environment.

Fig.1 The process of 3-fucosyllactose cell-free system construction. (CD BioGlyco)

• Identify the enzymes involved in the biosynthesis of 3-fucosyllactose. We need α1,2 fucosyltransferase (HpFutC) and α1,3 fucosyltransferase (HpFutA).
• Determine the order in which the enzymes act to synthesize 3-fucosyllactose. In this case, HpFutC will add the first fucose residue, followed by HpFutA adding the second fucose residue.
• Optimize and demonstrate full-length, soluble expression of HpFutC and HpFutA using CFPS. This involves setting up CFPS reactions with the appropriate DNA templates encoding the enzymes and the necessary cofactors and substrates.
• Mix the CFPS products containing HpFutC and HpFutA in the appropriate ratios to form an in vitro glycosylation (IVG) reaction. This reaction allows the enzymes to sequentially add fucose residues to lactose.
• Include the appropriate nucleotide-activated sugar donors (e.g., GDP-fucose) in the IVG reaction to provide the fucose residues for transfer by the enzymes.
• Purify the glycosylated product from the IVG reaction and analyze it using techniques such as mass spectrometry (MS) to confirm the presence of 3-fucosyllactose.

Applications of 2'-Fucosyllactose-based Cell-free System

• Researchers can use the 3-fucosyllactose-based cell-free system to investigate the functional properties of fucosylated glycans.
• Fucosylated glycans, including 3-fucosyllactose, are implicated in various diseases, including cancer and autoimmune disorders. The 3-fucosyllactose-based cell-free system can aid in the discovery of disease-specific glycan biomarkers for diagnostic and prognostic applications.
• Fucosylated glycans play a crucial role in xenotransplantation by mediating the interaction between xenograft tissues and the recipient's immune system. The 3-fucosyllactose-based cell-free system can contribute to studying and engineering glycan profiles in donor tissues to improve graft compatibility and reduce rejection rates.

Advantages

• CD BioGlyco boasts a team of experts well-versed in glycoscience and CFPS. Their deep understanding of glycan biosynthesis pathways and enzymatic reactions enables efficient construction of complex cell-free systems.
• With efficient project management practices in place, CD BioGlyco adheres to strict timelines and ensures timely delivery of constructed cell-free systems. This allows clients to advance their research and development activities without unnecessary delays.
• At CD BioGlyco, we promote collaboration by closely engaging with clients to comprehend their needs and objectives. This cooperative mindset guarantees that we not only meet but surpass client expectations, nurturing enduring partnerships for the long term. Clients can find our Custom Chassis Development Services on our website.

Similar Services at CD BioGlyco

At CD BioGlyco, we efficiently produce 3-fucosyllactose without the need for living cells. Contact us today to explore how our 3-fucosyllactose-based cell-free system construction service can accelerate your research and development in glycobiology and biotechnology. Moreover, CD BioGlyco also provides pre-produced Glycan Products for clients to choose from.

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