Specific Glycan Molecule Production Service
Synthetic Glycobiology-The Synthesis of Specific Glycan Molecule
Whether the client wants to use our custom strains or cells to complete the project, or to improve the production efficiency of large-scale glycans, our lab provides you with the most reliable solution. Whether the clients want to Generate Chassis or Develop Chassis, our leading technical team supports you all around. Based on synthetic glycobiology, CD BioGlyco provides accurate glycan molecular production service with the support of a professional team. Moreover, we provide various carbohydrate Production services as follows:
Specific Glycan Molecule Production Service at CD BioGlyco
Synthetic biology is an advanced mode of genetic manipulation that combines modularity and engineering that our researchers reprogram by changing molecular DNA. For the specific glycan molecule, our production team offers you a one-stop solution from host selection to optimized host development and scale production. Our lab has advanced instrumentation and experienced operators to meet clients' custom glycan production needs.
- Genetic engineering
- CD BioGlyco provides gene editing strain screening, and mutation services. We screen for the production of high-quality, high-yield key expression strains by adding DNA coding for enzymes and cloning the coding sequences of mutant genes into plasmids downstream of the promoter in a culture environment. Moreover, advanced strains are designed for specialized expression.
- Genetic modification
- We provide gene modification services on sequenced genomes to recode, synthesize, and insert DNA molecules from them into the host. Through a range of genome modification services including multi-copy integration, codon optimization, secretion signals, tunable promoter systems, post-processing, and folding mechanisms to improve specific functions or add new ones.
CD BioGlyco has a professional team of glycan production services to synthesize specific glycans according to client needs. We mainly provide specific glycan molecule production services:
Oligogalactose Production Service
Oligogalactose (GOS) is a class of prebiotic carbohydrates composed of galactose unit chains and terminal glucose. Its degree of polymerization (DP) is between 2 and 10. CD BioGlyco mainly provides two strategies to produce GOS including yeast fermentation strategy and enzymatic biosynthesis strategy. Our staff alters the structure of the GOS by controlling the fermentation conditions, and β-galactosidase and transgalactosylation reaction conditions.
Nanocellulose Production Service
Nanocellulose is a class of polymers composed of β-1,4-D-glucose monomers. Combining genetic engineering, metabolic regulation, and synthetic biology, we modify and design strains to produce high yields and specific structures of nanocellulose. These are the strains commonly used in our lab include Gluconacetobacter and Komagataeibacter family (such as K. xylinus, K. hansenii, K. rhaeticus, K. europaeus ,and K. medellinensis.). Strains of these species are the most potent producers of cellulose of all bacteria.
Rosavin Production Service
Rosavin is a special component of rose rhodiola and has a variety of medicinal activities. Rosavin are a family of cinnamyl mono- and diglycosides. With the support of glycosyltransferase engineering and artificial pathway construction, CD BioGlyco provides reliable high-titer rosavin production service through E. coli transformation engineering. Our development team optimizes the supply of the precursor UDP-arabinose using a two-step pathway consisting of α-arabinokinase and UDP-sugar pyrophosphorylase.
Salidroside Production Service
Salidroside is a precious phenylethanoid glycoside. Whether you want to express salidroside from E. coli, Saccharomyces cerevisiae or other strains, CD BioGlyco provides the most professional biosynthesis solution. We construct recombinant strains through metabolic engineering strategies, codon optimization, and expression pathway intervention. To increase yields, our R&D team dynamically regulates cell growth and product accumulation, intervening in cell and tissue culture, enzyme catalysis, and microbial metabolic engineering.
D-allulose Production Service
D-allulose is a natural rare sugar that has a wide range of uses in the food, pharmaceutical, and medical industries. CD BioGlyco provides high-quality D-allulose production services through E. coli, Bacillus subtilis or other strains. We engineer D-allulose 3-epimerase (DAEase) or D-psicose 3-epimerase (DPEase) by deleting fruA, manXYZ, mak, galE, and fruK to achieve co-expression. The recombinant strains we designed and constructed are more heat resistant, acid resistance, yield and titer.
Publication
Technology: Genetic engineering, Fluorescence microscopy, Genetic modification, Electroporation, RNA sequencing
Journal: Microbial biotechnology
Published: 2019
IF: 6.575
Results: In this study, lactose was used as a carbon source to express and produce nanocellulose in K. xylinus mutant with the help of genetic engineering. The cellulose produced using this system was 28-fold more than the wild-type strain. In addition, the researchers performed precise control by genetic modification of cellulose synthesis genes to produce cellulose of a specific structure.
Fig.1 Biosynthetic pathways for bacterial nanocellulose by K. xylinus and assembly of nanofibrils. (Jacek, et al., 2019)
Applications
- Nanocellulose is a biocompatible material that plays a key role in tissue engineering study and drug delivery systems development.
- Nanocellulose has superior immune potential and is widely used in the biomedical field.
- Based on nanocellulose, extensively developing antimicrobial materials including wound dressings, drug carriers, and packaging materials.
- GOS is instrumental in the development of new functional prebiotics.
Frequently Asked Questions
Why choose synthetic biology to produce specific glycan molecules?
Synthetic biology has been hailed as the third revolution in biotechnology, combining multiple glycobiological platforms such as genetic engineering and metabolic engineering to design and manufacture molecules with desirable properties. With the support of synthetic biology, it is possible to discover and improve synthetic pathways for specific molecules, replacing the original synthetic pathways (naturally derived or chemically synthesized). Chemical methods are suitable for the synthesis of carbohydrates with clear and simple structures, and too many steps easily affect the yield. In contrast, the synthesis of specific glycan molecules using synthetic biology is very fast and accurate. Through synthetic biology, the gene clusters that produce these metabolites are heterologously expressed and prepared on a large scale using fermentation engineering.
What are the modules of a synthetic biological system?
Synthetic biological systems are composed of several basic modules encoding gene switches, genome engineering, biocatalysts, and proteins. The basic building blocks assemble into polymers such as proteins and nucleic acids, which interact functionally to constitute pathways. The engineering of synthetic systems in biological entities enables synthetic biologists to exploit the capabilities and properties of cells to make valuable molecules.
CD BioGlyco has a mature production team, and a strict control system to provide clients with high-efficiency production service. Our serious service attitude and efficient service quality are recognized by clients around the world. Our staff always serve you with a philosophy of enthusiasm and professionalism. Please feel free to contact us for more information.
References
- Jacek, P.; et al. Molecular aspects of bacterial nanocellulose biosynthesis. Microbial biotechnology. 2019, 12(4): 633-649.
- Pázmándi, M.; et al. Production of high-purity galacto-oligosaccharides by depleting glucose and lactose from galacto-oligosaccharide syrup with yeasts. Yeast. 2020, 37(9-10): 515-530.
For research use only. Not intended for any clinical use.
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