Empowering Innovation with Corynebacterium glutamicum at CD BioGlyco

Corynebacterium glutamicum, a model industrial microorganism considered biologically safe, has not only been successfully used in the fermentation industry for large-scale production of amino acids but also has the potential to synthesize a variety of novel chemicals. At CD BioGlyco, we use synthetic biology to optimize Corynebacterium glutamicum chassis cells and construct Corynebacterium glutamicum engineering to help our clients expand the Bacterial Chassis production performance.

Chassis cell construction

Top-down (genome reduction) approach: Two insertion sequence (IS) elements are identified in Corynebacterium glutamicum, the knockdown of which improves the production properties of polyhydroxybutyric acid and γ-aminobutyric acid. In addition to the IS elements, we both simplify the genome and continue to maintain the productive properties of the cells by deleting prophage sequences and other unnecessary genes.

In addition to genome modification, we also offer modification of the intracellular "space" of Corynebacterium glutamicum. We construct bacterial microcompartments (BMCs) genes in Corynebacterium glutamicum to express BMCs, which further enhances the productivity of Corynebacterium glutamicum.

Genetic modification

• Knockout and Knock-in

  Based on suicide plasmids: Through two rounds of homologous recombination and screening, this method is widely used to genetically modify most positions of the chromosome.

  Clustered regularly interspaced short palindromic repeats (CRISPR)-based genome editing: This method is highly binding specific and also accomplishes re-editing of large segments of genes. At the same time, the method enriches the successfully edited cells and inhibits cell death through efficient reverse screening.

  In addition, we offer transcription activator-like effector nucleases (TALENs) for gene editing.

• Single-stranded DNA recombination

  We recombinantly edit the single-stranded DNA of Corynebacterium glutamicum by the CRISPR/Cas system. This method achieves hundreds of correctly edited clones at one time. In addition, we use this method to construct mutant libraries of individual loci of target genes and screen mutants with enhanced ability to release product feedback inhibition and exocytosis of glutamate.

Applications

• Corynebacterium glutamicum engineering can be used for large-scale production of amino acids and their derivatives.
• Corynebacterium glutamicum does not produce endotoxin and is a food-safe strain. Therefore Corynebacterium glutamicum engineering can be used to produce food-grade chemical additives.
• Corynebacterium glutamicum engineering can be used to modify nucleic acid molecules and proteins.

Advantages

• We have rapid experimental efficiency to ensure high quality and efficient fulfillment of clients' orders.
• We have a variety of first-class biological technologies or methods, such as Chassis Development, gene-directed editing, bioinformatics analysis, novel drug screening, etc.
• We provide high-quality client services, such as rapid response to client needs and customized services.

CD BioGlyco has an experienced service team, and we utilize the latest in synthetic biology technology to help our clients improve their Production of biomolecules and chemicals. Our services have been well received by our clients. If you have any questions, please feel free to contact us.

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