Chassis Strain Screening Service
The chassis strain screening service offered by CD BioGlyco involves the systematic evaluation and selection of microbial strains optimized for specific applications, particularly in glycan-related processes.
Chassis Strain Screening Service at CD BioGlyco
CD BioGlyco's chassis strain screening service is specifically designed for Glycan Production, offering a comprehensive approach to strain selection and optimization. Through the glycan production strain screening service, a wide array of microbial strains is evaluated using high-throughput methodologies to identify candidates with superior glycan production capabilities based on yield, purity, and structural fidelity. Following this, the glycan production strain isolation service isolates and purifies the selected strains, ensuring genetic stability and reproducibility. Finally, the glycan production strain cultivation service optimizes growth conditions and bioreactor parameters to maximize glycan yield and quality, providing clients with tailored microbial strains optimized for efficient glycan synthesis. Moreover, we also provide our clients with a wide range of Chassis Cells, based on which we can develop custom glycan engineering cells with our Development Services.
Glycan Production Strain Screening Service
The glycan production strain screening service offered by CD BioGlyco encompasses a comprehensive array of methodologies aimed at identifying and optimizing strains for efficient glycan production. This service includes high-throughput screening (HTS), which employs automated processes to rapidly analyze numerous strains for their glycan production capabilities. Additionally, genomics-based analysis delves into the genetic makeup of strains to identify potential markers associated with enhanced glycan production. Metabolomics-based analysis focuses on the metabolic pathways within strains to assess their capacity for glycan synthesis. Furthermore, proteomics-based analysis examines the protein expression profiles of strains to pinpoint key proteins involved in glycan biosynthesis.
Especially for the HTS service, we provide but are not limited to Fluorescence-activated cell Sorting (FACS)-based Strain HTS Service and Microfluidic System-based Strain HTS Service.
HTS Service for Chassis Strains
Genomics-based Analysis for Chassis Strain Screening
Metabolomics-based Analysis for Chassis Strain Screening
Proteomics-based Analysis for Chassis Strain Screening
Glycan Production Strain Isolation Service
The glycan production strain isolation service provided by CD BioGlyco offers a specialized solution for the isolation and selection of strains optimized for glycan production. This service involves the meticulous isolation of individual strains from a diverse pool of microbial cultures or genetically engineered organisms.
Glycan Production Strain Cultivation Service
CD BioGlyco utilizes state-of-the-art bioreactor systems and cultivation protocols tailored to the specific requirements of glycan biosynthesis. The cultivation process includes the optimization of various parameters such as temperature, pH, nutrient composition, and aeration to enhance the growth and glycan production capabilities of the strains. Continuous monitoring and analysis of key performance indicators enable real-time adjustments to the cultivation process, ensuring optimal conditions for glycan synthesis.
Publication Data
Technology: Genetic engineering, Synthetic biology, Golden gate cloning, CRISPR/Cas9 genome editing
Journal: Frontiers in Bioengineering and Biotechnology
IF: 5.7
Published: 2022
Results: The research discussed in the article focuses on using traditional fermentation processes and microbes as synthetic biology chassis to address future food challenges. The authors highlight the importance of understanding enzymes and enzyme complexes and their potential applications in genetic engineering. They discuss the use of biofoundries and laboratory automation to accelerate research in this field. The article also emphasizes the challenges in engineering non-model organisms and the need for a greater understanding of these organisms. The authors mention the use of modular tool-boxes and Golden Gate cloning as techniques for efficient genetic engineering. They also discuss the advantages of synthetic biology in constructing complex pathways and synthetic chromosomes. The article concludes by mentioning the potential of engineered microbes in producing future foods with benefits such as reduced water consumption, less land use, and improved organoleptic and nutritional properties.
Fig.1 Challenges of future foods which give rise to opportunities for synthetic biology. (Ramírez Rojas, et al., 2022)
Applications
- Screening chassis strains for industrial enzyme production is essential for identifying strains with robust enzyme activity, stability, and substrate specificity. Chassis strains are engineered to express recombinant viral antigens and glycoproteins for vaccine production.
- Chassis strain screening is fundamental in synthetic biology research for engineering microbes with novel metabolic pathways and genetic circuits. Chassis strains are engineered for the production of glycolipids, glycoproteins, and polysaccharides used as biological surfactants, emulsifiers, and biopolymers in various industrial applications.
Advantages of CD BioGlyco
- CD BioGlyco provides custom chassis strain screening services tailored to the specific needs and objectives of each client.
- CD BioGlyco employs multi-omics approaches, including genomics, metabolomics, and proteomics, to comprehensively assess chassis strain performance.
- CD BioGlyco values collaboration and maintains open communication with clients throughout the screening process.
At CD BioGlyco, our chassis strain screening service for glycan production offers a systematic approach to identifying, isolating, and cultivating microbial strains optimized for efficient and high-yield glycan synthesis. By combining screening, isolation, and cultivation services, clients can accelerate the development of robust microbial strains tailored to their specific glycan production requirements. Please do not hesitate to contact us for professional solutions and specialized quotes!
Reference
- Ramírez Rojas, A.A.; et al. Microbes of traditional fermentation processes as synthetic biology chassis to tackle future food challenges. Frontiers in Bioengineering and Biotechnology. 2022, 10: 982975.
For research use only. Not intended for any clinical use.
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