CD BioGlyco utilizes state-of-the-art techniques to modify and improve the glycosylation pathways in a variety of Host Cells, enabling them to produce Desired Glycoforms of therapeutic proteins and other glycan-related products. our services not only enhance the functionality and effectiveness of the products but also reduce the cost and time involved in the production process.
Our Comprehensive and High-quality Chassis Development Service
Through chassis strain development services, CD BioGlyco creates new, enhanced strains of organisms to meet production demands. We also offer chassis strain screening services to identify and isolate the most productive strains for industrial use.
Moreover, we also provide a range of optional services designed around synthetic biology and fermentation. One such service is the synthetic biology-based fermentation development service, which designs and develops fermentation processes that can optimize the productivity of the engineered cells. This can involve media development, fermentation operation, and process control, to name a few. Additionally, we offer a synthetic biology-based glycan separation and purification service, which involves the utilization of advanced techniques and methodologies to separate and purify glycans, a type of molecule commonly found in bio-therapeutics. Lastly, CD BioGlyco also provides a synthetic biology-based glycan modification service. This involves changing the structure of the glycans to create new, enhanced bio-therapeutics.
Fig.1 Comprehensive chassis strain development process, plus optional downstream services. (CD BioGlyco)
The development of chassis strain involves the engineering and alteration of microbial chassis to produce organisms that are compatible with synthetic biology functions. The objective is to generate a streamlined and efficient chassis capable of executing specified functions or generating desired goods.
Genome Editing Service for Chassis Development
Genome editing plays a crucial role in the development of synthetic microbial chassis for industrial applications. It allows for precise modifications of the genome to enhance the desired traits and functionalities of the chassis organisms. Several genome editing techniques have been widely used, including CRISPR-Cas9, TALENs, and zinc finger nucleases. CD BioGlyco provides several genome editing services for chassis development, this allows for the development of highly efficient and tailored microbial chassis that can be used for the production of pharmaceuticals, chemicals, and other valuable glycan products.
Multi-gene Editing Service for Chassis Development
Gene Family Editing Service for Chassis Development
Promoter Editing Service for Chassis Development
Off-targeted Genome Editing Service for Chassis Development
Targeted Genome Editing Service for Chassis Development
Genetic Code Expansion Service for Chassis Development
Strain Validation Service for Chassis Development
After implementing genome editing procedures, it is essential to validate the resulting strains to ensure that the desired genetic modifications have been successfully introduced and that the strains exhibit the expected phenotypic characteristics. Our strain validation service involves comprehensive analysis and validation of the edited strains through various molecular and phenotypic assays.
Sequencing Service for Chassis Validation
Chip Sequencing (Chip-Seq) Service for Chassis Validation
Next-generation Sequencing (NGS) Service for Chassis Validation
Our NGS services for chassis validation include but are not limited
Whole Genome Sequencing (WGS)-based Chassis Validation Service
RNA Sequencing (RNA-Seq)-based Chassis Validation Service
Targeted Sequencing-based Chassis Validation Service
CD BioGlyco offers comprehensive chassis strain screening services including chassis strain screening service, chassis strain isolation service, and chassis strain cultivation service. By leveraging our expertise in strain screening, isolation, and cultivation, we empower clients to identify, isolate, and cultivate chassis strains with enhanced performance characteristics, thereby accelerating the development and deployment of novel glycan biotechnological solutions and bio-based glycoproducts.
Glycan Production Strain Screening Service
Our chassis strain screening service involves the systematic screening of a diverse library of chassis strains to identify variants with desirable characteristics for specific applications. Through high-throughput screening assays and analytical techniques, we identify promising chassis strains that exhibit optimal performance metrics for further development and optimization.
High-throughput Screening (HTS) Service for Chassis Strains
Genomics-based Analysis for Chassis Strain Screening
Metabolomics-based Analysis for Chassis Strain Screening
Proteomics-based Analysis for Chassis Strain Screening
Our HTS services for chassis strains include but are not limited
Glycan Production Strain Isolation Service
Following the screening process, CD BioGlyco provides chassis strain isolation services to isolate individual strains or clonal populations with superior traits or desired genetic backgrounds. Our isolation techniques ensure the purity and genetic integrity of the isolated strains, enabling precise characterization and downstream applications.
Glycan Production Strain Cultivation Service
CD BioGlyco also offers chassis strain cultivation services to facilitate the scale-up and cultivation of selected chassis strains under controlled conditions. We provide tailored cultivation protocols optimized for the growth and productivity of specific chassis strains in various fermentation systems, including bioreactors, shake flasks, and microtiter plates. Our cultivation services enable the production of biomass or target products for downstream applications, such as enzyme production, metabolite synthesis, or bioprocess optimization.
CD BioGlyco offers a synthetic biology-based fermentation development service that encompasses a range of solutions to optimize and enhance fermentation processes. This service leverages principles of synthetic biology to engineer microbial strains and improve fermentation efficiency. Through this service, CD BioGlyco assists clients in achieving higher yields, faster production rates, and better overall performance in their fermentation processes.
Enzyme Development Service of Fermentation Development
This service focuses on the optimization and enhancement of enzymes used within the fermentation process. It involves the development of novel enzymes or the modification of existing enzymes to improve their efficiency, specificity, and stability in the fermentation environment.
Enzyme Artificial Intelligence Design Service
Structure-Guided Enzyme Development Service
Directed Evolution-based Enzyme Development Service
Fermentation Process Optimization Service
This service is dedicated to optimizing the fermentation process itself. It involves fine-tuning various parameters such as temperature, pH, nutrient concentrations, and fermentation duration to maximize product yields, minimize production costs, and ensure consistent product quality.
Optimization Service of Fermentation Media
Optimization Service of Fermentation Condition
Optimization Service of Nutritional Control
Optimization Service of Fermentation Kinetics
Optimization Service of Auxiliary System
CD BioGlyco offers comprehensive synthetic biology-based glycan separation and purification services, which include several specialized methods. These services cater to the diverse needs of glycan research and production, providing clients with advanced solutions for the isolation and purification of glycans for various applications.
Crystallization Technology-based Separation and Purification Service: Utilizing crystallization techniques to separate and purify glycans from complex mixtures.
Extractive Separation Technology-based Separation and Purification Service: Employing extractive separation methods to isolate and purify glycans efficiently.
Membrane Separation Technology-based Separation and Purification Service: Utilizing membrane separation technology for the selective separation and purification of glycans.
Distillation Technology-based Separation and Purification Service: Employing distillation techniques to separate and purify glycans based on their physical properties.
The subsequent modification services for polysaccharides produced using synthetic biology techniques include
Chemical Synthesis-based Glycan Modification Service: Chemical alteration of polysaccharide structures to introduce specific functional groups or properties.
Polymerization-based Glycan Modification Service: Polymerization techniques are applied to polysaccharides to alter their physical or chemical properties, such as molecular weight or chain structure.
Glycan-related Plastic Modification Service: Modification of polysaccharides to enhance their plasticity or suitability for specific applications, often through chemical or enzymatic methods.
Publication Data
DOI: https://doi.org/10.1093%2Fglycob%2Fcwac082
Technology: Combinatorial DNA assembly, Mass spectrometry analysis, Glycoconjugate sandwich ELISA, Densitometry
Journal: Glycobiology
IF: 4.3
Published: 2023
Results: The article presents a combinatorial DNA assembly approach for the biosynthesis of N-linked glycans in E. coli. The study demonstrates the successful production of glycoconjugates using this approach and quantifies the glycan abundance using sandwich ELISA. The authors also perform densitometry and mass spectrometry analysis to evaluate glycan production. The results show significant differences in glycan production between strains. This study provides a promising strategy for the synthesis of N-linked glycans and expands our understanding of glycoengineering in bacteria.
Fig.2 Schematic of the N-glycosylation pathway of C. jejuni. (Passmore, et al., 2023)
Advantages of Us
- CD BioGlyco specializes in glycoscience, offering deep expertise in the synthesis, modification, and analysis of glycans.
- CD BioGlyco provides a wide range of services, including glycan synthesis, modification, characterization, and analysis, catering to various research and industrial needs.
- CD BioGlyco offers tailored synthetic biology-associated solutions to meet specific client requirements, providing personalized services and support throughout the project lifecycle.
CD BioGlyco is recognized for its commitment to excellence, innovation, and customer satisfaction in the field of glycoscience. CD BioGlyco's chassis development service offers tailored solutions for the engineering and optimization of microbial host strains, enabling enhanced performance and productivity in bioproduction processes. Please do not hesitate to contact us for more detailed information or a personal quote.
Reference
- Passmore, I.J.; et al. A combinatorial DNA assembly approach to biosynthesis of N-linked glycans in E. coli. Glycobiology. 2023, 33(2): 138-149.
For research use only. Not intended for any clinical use.
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