Turning Kluyveromyces Engineering into Masterpieces

In recent years, the rapid development of synthetic biology has had a profound impact on life science research and industrial biotechnology. Especially in the construction of microbial cell factories to biosynthesize biofuels, chemicals, and other products, it shows great application prospects. Among them, Fungal Chassis Cells have many advantages as heterologous expression hosts, such as a clear genetic background, a strong ability to secrete proteins, and moderate glycosylation.

Synthetic biology-based Kluyveromyces Engineering Service at CD BioGlyco

Here, with cutting-edge synthetic biology technology, CD BioGlyco has constructed a comprehensive Chassis Cell Platform to provide high-quality Kluyveromyces engineering service, as well as a wide range of Glycobiomolecule Production Services to clients.

• Synthetic Biology Component

  CD BioGlyco builds on known metabolic networks and recombinant DNA technology to improve Kluyveromyces chassis cell performance by designing synthetic biology functional elements to precisely regulate metabolic pathways.

  • Promoter and terminator

    We increase the yield of exogenous products in Kluyveromyces chassis cells by constructing and screening for strong promoters and terminators.

  • Signal sequence

    The selection of suitable signaling sequences is an important part of the efficient synthesis of endogenous/exogenous proteins in Kluyveromyces chassis cells. We provide a stable and reliable solution for the efficient expression of heterologous proteins by developing universal and efficient secretion signal sequences.

  • Selection marker

    In the Kluyveromyces genetic manipulation system, we chose nutrient-deficient phenotypes and drug resistance as screening markers. Among them, the utilization of nitrogen-derived screening markers is an important specific screening scheme in Kluyveromyces. Such as URA3, LEU2, and TRP1 are ideal markers for screening Kluyveromyces positive transformants.

• Gene Editing Strategies

  During metabolic engineering and synthetic biology modification, genetic modification of multiple genes is usually involved. Therefore, efficient gene editing technologies are essential tools for metabolic engineering and synthetic biology.

  • Targeted gene integration technology

    Integration of exogenous DNA fragments into the genome of microbial chassis cells requires the aid of its double-strand break (DSB) repair mechanism. These include both homologous recombination and non-homologous end-joining repair mechanisms.

    • Homologous reorganization

      Homologous recombination integration of exogenous genes mainly relies on the RAD52 gene cluster, including the genes RAD50, RAD51, RAD52, and XRS2, which can achieve precise targeted integration of exogenous genes in the chassis cell genome.

    • Non-homologous end joining

      We improve the efficiency of Kluyveromyces heterologous gene targeting by knocking down the KU80 gene that mediates the non-homologous end joining of heterologous genes.

Applications

• Kluyveromyces engineering can be used to probe the ability of fungal chassis cells to secrete proteins.
• Kluyveromyces engineering can be used to modify Kluyveromyces strains and assist in the production of biomolecules such as lipids.
• Kluyveromyces engineering can be used to construct microbial cell factories.

Advantages

• We have an advanced chassis cell engineering platform for precise Chassis Cell Transformation and Modification.
• We integrate technologies from various fields such as biology, chemistry, and pharmacology to provide efficient and customized services to our clients.
• We focus on cost-effective solutions to ensure that our clients receive attentive service.

CD BioGlyco has rich experience in the field of synthetic biology and is capable of performing comprehensive chassis cell designs and applications. We are committed to providing multiple experimental programs to meet our clients' R&D needs. Please feel free to contact us and we will answer any questions you may have.

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