Pioneering a New Era in Streptomycetaceae Engineering

Streptomycetaceae is one of the most diverse and commercially valuable taxa-producing antibiotics, and the Streptomycetaceae genome contains an extremely rich resource of natural product biosynthesis gene clusters, which makes Streptomyces chassis cells uniquely suited as heterologous expression hosts. Based on the principles of synthetic biology, CD BioGlyco conducts research and Development of Chassis Cells to prepare heterologous high-yielding chassis strains and use them for the Production of more active compounds.

Comprehensive and Professional Streptomycetaceae Engineering at CD BioGlyco

Here, we provide professional Streptomycetaceae engineering service with our high level of multidisciplinary skills.

Fig.1 Schematic diagram of Streptomyces chassis construction. (CD BioGlyco)

Genome streamlining and optimization

We utilize the minimal genome to serve as a chassis microbe for heterologous expression. Second, we eliminate competition for substrate, energy and reducing power in the Streptomycetaceae metabolic pathway by knocking out the original secondary metabolite biosynthesis gene cluster, which reduces the difficulty of detection and purification of the heterologous product and enhances the yield of the heterologously expressed target.

Among other things, we offer a variety of knockout tools to our clients:

• Clustered regularly interspaced short palindromic repeats (CRISPR) system

• Transcription activator-like effector nucleases (TALENs)

• Others

Modification of regulatory factors

We use gene editing technologies to modify (knockout, replacement, etc.) regulatory factors to silence or overexpress pre-existing biosynthetic gene clusters in Streptomycetaceae. This approach eliminates metabolite interference effects or improves heterologous expression yields and optimizes chassis cell performance.

For example, we replace the original promoters in the Streptomycetaceae gene cluster with promoters induced by other microorganisms, resulting in improved yields and optimized heterologous expression systems for Streptomycetaceae.

Increased supply of precursors

We alter the metabolic pathways of Streptomycetaceae by combinatorial biosynthesis to increase substrate and precursor content. For example, we optimize the heterologous expression of target products by expressing genes related to enzymes in the Streptomycetaceae chassis. Knocking down specific genes of the enzyme to increase the enzyme level enhances the precursor supply for heterologous expression of the target product.

Applications

• Streptomycetaceae engineering can be used to design and modify Streptomycetaceae to enhance the ability of Streptomycetaceae to produce compounds.
• Streptomycetaceae engineering can be used to advance the process by which Streptomycetaceae chassis cells generate secondary metabolites of broad application value.
• Streptomycetaceae engineering can be used to probe the Bacteria Chassis Cell's metabolic regulatory system.

Advantages

• Professional technology: We have a strong Chassis Cell Platform, specializing in genomics, transcriptomics, and metabolic engineering technologies to provide our clients with professional biological services.
• Reliability and stability: Our experimental solutions and products are highly reliable and stable, and each of them has passed strict quality tests.
• Customized service: Our professional service team provides personalized solutions according to the client's specific needs.

As a recognized expert in the field of synthetic biology, CD BioGlyco has a dedicated research team and has executed many projects based on synthetic biology. Please feel free to contact us if you have any needs in this area.

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