Designing a Genetic Future with Saccharomyces cerevisiae at CD BioGlyco

Saccharomyces cerevisiae, as the first model eukaryotic organism sequenced in the whole genome, has the advantages of clear genetic background, strong genetic manipulation, and excellent fermentation performance. In addition, Saccharomyces cerevisiae can also be used as a Fungal Chassis for synthetic biology. As a professional biology research company, CD BioGlyco has extensive experience in the field of synthetic biology and has constructed a specialized Chassis Cell Platform to provide high-quality Saccharomyces cerevisiae engineering service.

• Metabolic Engineering of Saccharomyces cerevisiae

  Saccharomyces cerevisiae is the first model eukaryote to have its entire genome sequenced, and it is also one of the preferred chassis cells for metabolic engineering. At CD BioGlyco, we develop synthetic biology-guided metabolic engineering of Saccharomyces cerevisiae for Chassis Cell Optimization and Regulation to enhance Saccharomyces cerevisiae's ability to act as a cellular factory and to increase cellular target product yields.

• Portfolio pathway optimization

  We developed a customized optimization of metabolic pathways by combinatorial transcriptional engineering in Saccharomyces cerevisiae. In other words, by creating a series of promoter mutants with different strengths and using the obtained promoter mutants to regulate the expression levels of multiple genes in the target pathways at the same time, we generate pathway mutant libraries with multiple expression levels through DNA assembly and thus achieve combinatorial pathway optimization.

• Metabolic pathway enzyme co-localization

  Heterologous pathways in Saccharomyces cerevisiae require multiple enzymes to co-catalyze them, and CD BioGlyco utilizes metabolic pathway enzyme co-localization strategies to optimize these metabolic pathways.

  Examples include protein fusion expression, protein scaffold assembly, and subcellular structural localization, among other strategies. These strategies optimize the spatial distribution of key enzymes in metabolic pathways, thereby increasing the efficiency of substrate-enzyme interactions and reducing the metabolic flow of intermediates to competing pathways.

• Dynamic regulation engineering

  CD BioGlyco applies artificial dynamic regulatory systems based on Saccharomyces cerevisiae cellular metabolites or external input signals such as temperature and light to metabolic engineering in Saccharomyces cerevisiae.

  For example, malonyl coenzyme A is a key intermediate in fatty acid biosynthesis, and we have achieved flux control of malonyl coenzyme A and fatty acids in Saccharomyces cerevisiae through hierarchical dynamic pathway regulation to increase the yield of 3-hydroxypropionic acid.

Fig.1 Metabolic engineering of Saccharomyces cerevisiae. (CD BioGlyco)

• Saccharomyces cerevisiae Gene Editing Technologies

  CD BioGlyco applies advanced gene editing technologies to a wide range of applications in the design and construction of Saccharomyces cerevisiae cell factories. Examples include recombinase and homologous recombination-based gene editing technologies.

• Classical gene editing technology

  CD BioGlyco utilizes homologous recombination and screening marker methods for gene editing in Saccharomyces cerevisiae. In particular, homologous recombination technology utilizes undamaged homologous DNA fragments as templates for the addition, replacement, or inactivation of target genes.

• Meganuclease (MegNs) technology

  MegNs are deoxyribonucleic acid endonucleases characterized by large recognition sites that recognize double-stranded DNA sequences of more than 14 base pairs, such as the 18 bp sequence recognized by the homing enzyme in Saccharomyces cerevisiae. CD BioGlyco improves the efficiency of gene editing by introducing the homing enzyme recognition site into the chromosomal target location of the host.

Applications

• Saccharomyces cerevisiae engineering can be used in the biosynthesis of natural products. Examples include alkaloids, aromatic amino acids, terpenoids, and flavonoids.
• Saccharomyces cerevisiae is one of the preferred chassis cells for metabolic engineering. Therefore, Saccharomyces cerevisiae engineering can be widely used in the biomanufacturing of chemicals and novel high-value-added bioactives.
• Saccharomyces cerevisiae has the advantages of a clear genetic background, strong genetic manipulation, and good fermentation performance, therefore, Saccharomyces cerevisiae engineering can be applied in brewing and food production.

Advantages

• We offer custom Saccharomyces cerevisiae engineering services based on our client's research needs. For example, many types of gene editing.
• We provide one-stop Saccharomyces cerevisiae engineering services to our clients. In addition to the Production of the target compounds, we also carry out further research and development work, including performance testing of the products and optimization of the technology.
• We combine a multidisciplinary approach to Saccharomyces cerevisiae engineering, and our Saccharomyces cerevisiae engineering services can be applied to a wide range of fields, including biology and food.

CD BioGlyco thoroughly researches synthetic biology and provides comprehensive synthetic biology services to clients. We have developed various types of cell chassis to support our clients to complete their synthetic biology research efficiently. If you are interested, please feel free to contact us.

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