Aspergillus Engineering Service
With the development of synthetic biology and gene editing, CD BioGlyco uses metabolic engineering to design and construct GlycoChas™ Cells. Strain alteration occurs through random mutation and screening processes and provides a large scope for modification. The requirement for high yield, potency, and productivity is critical for the selection and construction of Fungal Chassis. We modify the genome to construct Aspergillus engineering strain by modulating a complex network of pathways for metabolic and transcriptional regulation. Our professional operators have rich experience in Chassis Development and Production services to help clients avoid detours.
Unlocking the Power of Aspergillus Engineering
Aspergillus species
CD BioGlyco provides a variety of high-quality Aspergillus e.g. A. niger, A. fischerianus (Neosartorya fischeri), A. oryzae, and A. terreus, A. flavus, and A. fumigatus. Our researchers screen the most suitable strains as hosts according to the needs of clients.
Transformation system of Aspergillus
The construction of an efficient genetic transformation system is a prerequisite for gene regulation and recombinant gene expression in Aspergillus. CD BioGlyco provides polyethylene glycol (PEG)/cacl2-mediated protoplast transformation system construction services.
Gene expression regulatory elements
Accurate gene expression is critical for synthetic biology. CD BioGlyco provides multi-transformation system constructs containing multiple selectively tagged gene-deficient strains to help customers knock in or delete multiple genes in the same host. We achieve the purpose of integrating polygenic intervention or deletion by controlling key components such as promoter, terminator, and selective marker genes. Moreover, we use several marker genes including the carbon toxin resistance marker gene (AosdhB (cxr)), pyrG, argB, niaD, thiamineresistance marker gene (thil), sC, amdS and pyrithione resistance gene (ptrA), and the bleomycin resistance marker gene(Blmb).
Genome engineering
We provide highly efficient targeted gene modification services by transcription-like activator effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeat sequences/CRISPR-associated nucleases 9 (CRISPR/Cas9).
Publication
Technology: Reverse phase chromatography, Nuclear magnetic resonance (NMR) spectroscopy, X-ray crystallography, Electrospray ionization high-resolution tandem mass spectrometry (ESI-HRMS/MS)
Journal: Fungal Biol Biotechnol
Published: 2019
IF: 2.91
Results: This study constructed a high-titer Aspergillus niger expression host and demonstrated for the first time that Aspergillus niger is a reliable and useful host for non-ribosomal peptide expression. The researchers heterologously expressed the non-ribosomal peptide synthetase ESYN in Aspergillus niger. The encoding gene, esyn1, was subjected to tunable bacterial-fungal hybrid promoter (Tet-on) regulation, and the promoter was initiated at the early culture stage. The metabolites were further separated using reverse phase chromatography and characterized with the support of X-ray, NMR, and MS/MS.
Fig.1 ESI-HRMS/MS analysis of enniatins produced by strain. (Richter, et al., 2014)
Applications
- Aspergillus engineering strain is often used in the enzyme industry to produce many natural and heterologous enzymes such as glucose oxidase, lysozyme, and lactase.
- Aspergillus engineering strain can be used to develop drugs for treating diseases.
- Aspergillus engineering strain can be used to produce citric acid.
Frequently Asked Questions
- Why choose Aspergillus as an ideal host for natural product pathway analysis and compound production?
- Aspergillus has a powerful and stable polymer degrading enzyme system that efficiently hydrolyzes a wide range of polymers.
- Aspergillus maintains long-lasting stability in extreme environments such as strong acids, far surpassing other microorganisms and effectively reducing the risk of contamination.
- Aspergillus has an endogenous phosphopantetheinyl transferase (PPTase) that plays a key role in the post-translational activation of nonribosomal peptide-synthetase (NRPS). As a fungus, its ability to synthesize secondary metabolites is conserved and subject to multiple regulatory interventions.
- How to overcome the limitations in transcription, translation, protein folding, translocation, degradation, transportation, and secretion in the production of exogenous proteins by strains?
With the deciphering of the full sequence of the strain genome, the bottleneck of heterologous production is solved by optimizing the genetic transformation system, constructing integration vectors, developing fusion expression strategies, optimizing the exogenous gene expression system, improving the efficiency of homologous recombination, utilizing mycelial fusion technology, and CRISPR/Cas9 genome editing system.
Combining genomic information, modern bioinformatics methods, and effective molecular genetic manipulation tools, CD BioGlyco constructs Aspergillus engineering as a fungal chassis to increase yield and potency. Please feel free to contact us for more information.
References
- Richter, L.; et al. Engineering of Aspergillus niger for the production of secondary metabolites. Fungal Biol Biotechnol. 2014, 1: 4.
- Tong, Z.; et al. Systems metabolic engineering for citric acid production by Aspergillus niger in the post-genomic era. Microb Cell Fact. 2019, 18(1): 28.
For research use only. Not intended for any clinical use.
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