Client Satisfaction Is the Driving Force of Our Work Related to Nannochloropsis oculata Engineering

CD BioGlyco builds on advances in trait mining, genetic engineering, and synthetic biology to design and construct a variety of Microalgae Chassis suitable for specific product Production, including Chlamydomonas reinhardtii, Dunaliella salina, N. oculata, etc. N. oculata is a good model for metabolic studies and a Chassis for synthetic biology. We provide a professional one-stop N. oculata engineering service for our client's research and Chassis Development needs. Based on various bioinformatics analyses, targeted gene disruption, gene inhibition, and other techniques, we achieve gene-specific N. oculata transformation, superior growth, and higher yield of target products.

• N. oculata engineering
  The development of genetic tools, bioinformatics tools, and computer prediction capabilities facilitates the design and manipulation of the N. oculata genome to develop good N. oculata. We design and construct specific N. oculata systems for the production of various types of substances such as glycoproteins through synthesis, recombination, and modification of biological genes.
  
  • Genome design: We characterize gene regulatory pathways, predict the outcome of metabolic changes, and perform functional annotation through a variety of bioinformatics tools and computer predictions.
  • Construction of N. oculata systems: We construct complete, study-specific N. oculata systems through gene up-regulation, down-regulation, knockout, and suppression strategies.
  • Validation of N. oculata mutants
• Gene editing tools for N. oculata engineering
  • Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas
  • Transcription activator-like effector nuclease (TALAN)
• What do we deliver?
  • Detailed lab report
  • Modified N. oculata system
  • Target products

Fig.1 Flowchart of our N. oculata engineering. (CD BioGlyco)

Frequently Asked Questions

• What are the advantages of N. oculata for synthetic biology?
  • Fast growth
  • Ease of transgenic manipulation
  • Short time to scale
  • Capable of post-translational modifications
  • Simple to culture
• What role does CRISPR/Cas play in N. oculata engineering?
  • Targeted knockout of genes by CRISPR/Cas 9
  • Targeted knock-in of genes by CRISPR/Cas 9
  • Up-regulation of gene expression by CRISPRa
  • Inhibition of target gene expression by CRISPRi

Applications

• N. oculata engineering is utilized for the verification of various gene functions, genetic screening, etc.
• N. oculata engineering is used for the production of various antibodies, recombinant glycoproteins, and other high-value products.
• N. oculata engineering is used to investigate the relationship between glycoprotein structure and activity.

Advantages of Us

• We improve the ability of N. oculata to target the production of target products by adding necessary genetic competence to N. oculata and removing competing pathways or unwanted product formation.
• Codon optimization is critical for high transgene expression. We optimize sequence optimization algorithms for N. oculata engineering.
• We have a professional research team and advanced equipment to support N. oculata engineering service.

CD BioGlyco combines traditional methods with emerging molecular genetics toolkits to provide a high-quality N. oculata engineering service. Welcome to contact us to learn more about N. oculata engineering.

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