Arabidopsis thaliana Engineering Service

Arabidopsis thaliana Engineering Service

Arabidopsis thaliana Engineering Service at CD BioGlyco

Arabidopsis thaliana, as a model organism, produces complex N-glycans that cannot be synthesized by E. coli or S. cerevisiae, making it an important organism commonly used in synthetic glycobiology strategies. A. thaliana engineering in synthetic glycobiology has emerged as a cutting-edge biotechnological approach that combines gene editing and biochemical alterations to alter the production of glycoproteins and other glycans. The process fundamentally involves the integration of multidisciplinary approaches from different fields, including biotechnology such as molecular biology, genetic engineering, biochemistry, and cell biology. CD BioGlyco has built the advanced GlycoChas™ Cells platform to offer a variety of chassis. As one of the important Plant Chassis offered by us, we offer high-quality A. thaliana engineering services to our clients.

A. thaliana engineering service. (CD BioGlyco)

  • Gene editing technology

    We use gene editing technologies such as the CRISPR/Cas9 system and transcription activator-like effector nucleases (TALENs) to manipulate the Arabidopsis genome.

    • CRISPR-Cas9 system: This is currently the most popular gene editing technology and can be used to precisely cut specific DNA sequences in the genome of A. thaliana. By designing specific RNA guide sequences, CRISPR-Cas9 accurately identifies and cuts target genes, thereby achieving gene knockout, insertion, or replacement.

    • TALENs technology: TALENs is another gene-editing technology based on DNA cutting. Similar to CRISPR-Cas9, TALENs cut DNA precisely at specific locations, but they are relatively more complex in design and construction.

  • Metabolic pathway modification technology

    We modify the metabolic pathways of A. thaliana by regulating or introducing new enzymes to produce specific glycans or glycoproteins. This could be used to alter the plant's cellulose content or introduce new pathways to produce glycans that are not naturally produced by the plant. Metabolic pathways commonly modified in this type of engineering include:

    • Glycosylation pathway: This process adds glycans to proteins or glycoproteins, modifying them to produce specific glycans with desired properties.

    • Cellulose biosynthesis: This pathway is modified to alter the cellulose content of the plant, thereby affecting its growth and other characteristics.

    • Other plant natural glycan biosynthesis: Pathways related to the synthesis of plant natural glycans such as sucrose, fructose, and glucose can be modified to change glycan content and distribution.

In addition, A. thaliana can also be made to produce glycoproteins by modifying specific genes and metabolic pathways. These glycoproteins can be used in drug development, biosensors, biomaterials, and other fields.

Publication

Technology: Structure-based enzyme engineering

Journal: Biochemical Journal

IF: 3.766

Published: 2020

Results: The authors utilized advanced molecular modeling and simulations combined with biochemical characterization to craft a study flow for five Group H A. thaliana UDP-dependent glycosyltransferases (UGTs), as crystal structures were not available experimentally. Through strategic structural alteration and analysis, they were able to pinpoint critical amino acids that, upon mutation, led to improved donor substrate recognition compared to the wild-type UGTs. Structural differences driving substrate preferences were identified through molecular dynamics simulations and deep learning analysis.

Here are some of the results shown in this article:

Fig.1 Structural profiles.Fig.1 Structural profiles of UGTs 76E1 and 76E5 against the three sugar substrates. (Akere, et al., 2020)

Advantages of Us

  • Technical expertise: We have profound biotechnology and gene editing expertise to provide clients with professional A. thaliana engineering services, such as gene editing and metabolic pathway optimization, to ensure the accuracy and reliability of experimental results.
  • Professional team: Our professional team provides a full range of services from gene cloning to plant transformation to meet the diverse needs of clients.
  • Customized services: We provide customized A. thaliana engineering solutions based on the specific needs of clients. We provide personalized service solutions based on client needs whether it is the modification of metabolic pathways, the creation of new traits, or the production of specific carbohydrate compounds.

CD BioGlyco focuses on providing comprehensive A. thaliana engineering services. We have advanced gene editing and metabolic engineering technologies to customize chassis organisms for clients and produce diversified glycan and glycoprotein products. These products include monosaccharides and polysaccharides such as glucose and sucrose, as well as glycoproteins with specific functions such as glycosylases. Please feel free to contact us if you are interested in our A. thaliana engineering service.

Reference

  1. Akere, A.; et al. Structure-based enzyme engineering improves donor-substrate recognition of Arabidopsis thaliana glycosyltransferases. Biochemical Journal. 2020, 477(15): 2791-2805.
For research use only. Not intended for any clinical use.
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