Penicillium Engineering Service
Penicillium Species-Chassis for Biomanufacturing
Fungal such as Aspergillus, Penicillium, Trichoderma, etc. are widely used as high-quality hosts for products such as proteins and secondary metabolites. CD BioGlyco provides professional GlycoChas™ Cells construction, Chassis Development, and Production services to clients relying on advanced technologies. Penicillium is widely found in air, soil, animal gut, plant tissues, and microorganisms. We provide highly efficient Penicillium engineering services by modulating the biosynthetic pathways and metabolic genetics of Penicillium. Moreover, we provide several chassis e.g. Plant Chassis, Microalgae Chassis, Insect Chassis, Bacteria Chassis, and Mammalian Chassis.
Your Reliable Partner for Penicillium Engineering
- Strain optimization
Improving Penicillium strain activity and yield is important in constructing fungal chassis. Microorganisms rely on genes to encode their activity information, and CD BioGlyco uses genetic manipulation to achieve optimization of strain activity. Moreover, we provide services for the optimization of strain culture conditions, taking care to analyze the influencing factors.
- Construction and verification of engineered Penicillium strains
Most secondary metabolite-related genes are silent or poorly expressed in Penicillium, We use improved homologous recombination and clustered regularly interspaced short palindromic repeat sequences/CRISPR-associated nucleases 9 (CRISPR/Cas9) as a genome editing tool to enable specific gene expression or deletion.
Our lab uses promoters and terminators as building blocks and more complex regulatory devices to control gene expression. We significantly express enhanced genes by modulating transcription factors utilizing strong promoters. For designed strains (deleted or overexpressed genes), we offer polymerase chain reaction (PCR) validation services.
Fig.1 Schematic diagram of the regulatory targets of the designed engineered strains. (CD BioGlyco)
Publication
Technology: RNA-sequencing, Reverse transcription-quantitative PCR (RT-qPCR), Random mutagenesis, Genetic engineering
Journal: Microbial Cell Factories
Published: 2022
IF: 6.4
Results: This study confirmed that genetic engineering and random mutagenesis were effective in increasing raw starch-degrading enzyme (RSDE) production. The researchers isolated a total of 3,619 mutated P. oxalicum colonies using γ-ray mutagenesis with strain A2-13 as the parental strain. Among them, mutant TE4-10 was simultaneously deficient in transcriptional repressor gene PoxCxrC and activator gene PoxAmyR overexpression by gene regulation. The yield of the modified engineered strain increased by 15.6% relative to TE4-10.
Fig.2 Analysis of gene expression in engineered P. oxalicum strain relative to the parent strain. (Zhao, et al., 2022)
Applications
- In food production, Penicillium is one of the most commonly used fungi for food and feed processing. It can be used as a leavening agent or as a fungal stock in cheese-making plants to add flavor and give desired color to foods.
- In the pharmaceutics and pharmaceutical industry, Penicillium engineering strains can be used for the production of bioactive metabolites in antimicrobial therapy, secondary metabolisms for anticancer and antiproliferative therapy, and bioactive metabolites with antidiabetic activity.
- Penicillium engineering strains play an important potential in the green synthesis of nanoparticles.
Advantages of Us
- The engineering services we provide not only help to reduce mycotoxins but also develop safe pathways for their induced transformation and expression.
- We use CRISPR/Cas9 with high flexibility and precision in target selection to ensure a high success rate of genetic engineering.
CD BioGlyco uses fungal strain to rapidly achieve the high-level production of a compound. Please feel free to contact us if you are interested in Penicillium engineering.
References
- Guzmán-Chávez, F.; et al. Engineering of the filamentous fungus Penicillium chrysogenum as cell factory for natural products. Frontiers in microbiology. 2018, 9: 2768.
- Zhao, S.; et al. Combination of genetic engineering and random mutagenesis for improving production of raw-starch-degrading enzymes in Penicillium oxalicum. Microbial Cell Factorie. 2022, 21(1): 272.
For research use only. Not intended for any clinical use.
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