H. polymorpha-Methylotrophic Yeast

The use of fungi as a chassis for the construction of GlycoChas™ Cells has gained tremendous support in recent years. Recombinant DNA allows the introduction of exogenous genes into a host organism to produce biologically active heterologous proteins. Therefore, the choice of host organism is critical. CD BioGlyco uses H. polymorpha as an expression platform to design recombinant strains through metabolic engineering and synthetic biology to produce the desired compounds. Our lab provides reliable and high-quality Chassis Development and Production services to clients. Besides H. polymorpha, we also offer a variety of fungal hosts as listed below based on our Fungal Chassis

High-quality H. polymorpha Engineering Service That Exceeding Your Expectations

• H. polymorpha Strains

  CD BioGlyco provides various H. polymorpha strains including CBS4732, LR9 odc1 derivative of CBS4732, RB11 odc1 derivative of CBS4732, NCYC495, ku80 and DL-1. These strains come from different sources and therefore exhibit different characteristics and chromosome numbers. Our staff recommends the most suitable strain for you based on the characteristics of your project.

• Gene Regulation

  CD BioGlyco achieves the goal of expressing multiple heterologous genes in a single strain by introducing new biosynthetic pathways such as gene introduction and deletion into the H. polymorpha. We perform genome editing mainly by clustered regions of interspersed palindromic repeats-CRISPR associated protein 9 (CRISPR/Cas9), which not only removes endogenous genes from an organism but also inserts exogenous sequences into its genome. H. polymorpha has different regulatory mechanisms, we provide services for the development of specific regulatory gene expression strategies.

  In addition, we offer specialized modification services. We use alternative promoters or extended incubation times to ensure Cas9 activity. Moreover, we ensure gene deletion rate at different loci by constructing integration plasmids.

• Strain Culture

  For H. polymorpha strains, we optimize the culture conditions by testing different media compositions, pH, and other factors that may affect the desired protein productivity.

Fig.1 Schematic diagram of genetic tools in H. polymorpha. (CD BioGlyco)

Frequently Asked Questions

• Why use the H. polymorpha as a host for heterologous expression?
  • H. polymorpha contains a variety of strong inducible promoters including key enzymes of methanol metabolism, and a strong promoter of cell membrane-bound ATPase. Moreover, it also contains several very strong structural promoters such as glyceraldehyde 3-phosphate dehydrogenase (GAP) and translation elongation factor 1-α (EF1α).
  • H. polymorpha has heat resistance, a large range of pH tolerance, and good fermentation characteristics. H. polymorpha secretes very small amounts of endogenous proteins that promote downstream processing.
  • H. polymorpha can be easily constructed into engineered strains for protein production and has shown human-like N-glycosylation of secreted proteins.
  • H. polymorpha effectively avoids hyperglycosylation of recombinant proteins compared to other yeasts.

• What are the differences between the common H. polymorpha strains?

CD BioGlyco produces the desired compounds by controlling the biosynthetic pathway and transferring it to a suitable host. Our professionalism and excellent quality of service are recognized by our clients. Please feel free to contact us for more infromation.

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