Chassis Strain Development Service
Through meticulous design and selection of Chassis Strains, CD BioGlyco aims to enhance the efficiency, yield, and quality of Glycan production processes. Additionally, the service involves screening and validation steps to ensure the robustness and reliability of the engineered strains for industrial-scale glycan production.
Chassis Strain Development Service Including Genome Editing and Validation
CD BioGlyco's chassis strain development services encompass comprehensive support for the optimization and validation of microbial strains intended for various applications, particularly in the realm of glycan-related products. This service includes genome editing service for chassis development, which involves precise genetic modifications to tailor the chassis strain for specific glycan biosynthesis pathways. Additionally, the strain validation service for chassis development ensures the integrity, stability, and functionality of the engineered strains, validating their suitability for efficient glycan production processes.
Fig.1 Chassis engineering for microbial production. (CD BioGlyco)
Genome Editing Service for Chassis Development
CD BioGlyco's genome editing service for chassis development involves sophisticated techniques to precisely engineer microbial genomes for optimized glycan production. This service leverages cutting-edge genome editing tools such as CRISPR-Cas9, TALENs, or zinc finger nucleases to introduce targeted modifications into the chassis strain's genetic makeup. These modifications may include gene knockouts, knock-ins, point mutations, or gene insertions, strategically designed to enhance glycan biosynthesis pathways, improve metabolic flux, or eliminate unwanted byproducts. CD BioGlyco's team of experts employs state-of-the-art molecular biology techniques to design, execute, and validate these genomic edits, ensuring accuracy, efficiency, and safety throughout the process.
Multi-gene Editing Service for Chassis Development
Gene Family Editing Service for Chassis Development
Promoter Editing Service for Chassis Development
Off-targeted Genome Editing Service for Chassis Development
Targeted Genome Editing Service for Chassis Development
Genetic Code Expansion Service for Chassis Development
Strain Validation Service for Chassis Development
This service encompasses comprehensive validation protocols designed to assess the performance and characteristics of the genetically modified chassis strains. Through a combination of phenotypic and genotypic analyses, CD BioGlyco evaluates key parameters such as growth kinetics, glycan yield, genetic stability, and product consistency. Validation studies may include fermentation trials, high-throughput screening assays, genomic sequencing, and molecular profiling to confirm the intended genetic modifications and assess the strain's overall suitability for large-scale glycan production. By rigorously validating the engineered strains, CD BioGlyco ensures the delivery of robust and reliable chassis platforms optimized for glycan biosynthesis applications.
Sequencing Service for Chassis Validation
Chip Sequencing (Chip-Seq) Service for Chassis Validation
Next-generation Sequencing (NGS) Service for Chassis Validation
The NGS service, which includes but is not limited
- Whole Genome Sequencing (WGS)-based Chassis Validation Service
- RNA Sequencing (RNA-Seq)-based Chassis Validation Service
- Targeted Sequencing-based Chassis Validation Service
Publication Data
Technology: Gene deletion/overexpression, Synthetic biology, Synthetic genomes, Artificial transcription/translation systems, Genome-scale engineering or editing tools, Bottom-up reconstruction of cellular machineries, Gene circuits
Journal: Current Opinion in Biotechnology
IF: 7.7
Published: 2020
Results: The research discusses the importance of chassis engineering in microbial production of chemicals and bioproducts. It explains that chassis engineering involves modifying the genetic makeup of organisms to optimize their ability to produce desired products. The traditional approach involves gene deletion/overexpression to regulate precursor and product transportation. However, with the development of synthetic biology, chassis engineering has expanded to include the synthesis of artificial cellular machineries. This allows for the creation of synthetic cells that can produce bioproducts. The text also mentions the challenges and limitations of synthetic chassis engineering, such as the high cost of construction and the need for a better understanding of cellular physiology and metabolism. Overall, the development of chassis engineering technologies is expected to advance the design and construction of customized chassis for the production of target bioproducts.
Applications
- Engineered chassis strains are utilized for the production of therapeutic glycoproteins.
- Chassis strains are engineered to express recombinant viral antigens and glycoproteins for vaccine production.
- Glycan-related products development such as prebiotics, probiotics, and dietary fibers are produced using engineered chassis strains.
- Chassis strains are engineered for the production of glycolipids, glycoproteins, and polysaccharides used as biological surfactants, emulsifiers, and biopolymers in various industrial applications.
- Engineered chassis strains serve as platforms for the production of glycan-based diagnostic reagents, research tools, and assay components.
Advantages of CD BioGlyco
- CD BioGlyco specializes in glycoscience and has deep expertise in the development of chassis strains for glycan-related applications. Our team consists of scientists with extensive knowledge in glycan biosynthesis, glycoengineering, and strain optimization.
- CD BioGlyco provides tailored solutions to meet the specific requirements of clients.
- CD BioGlyco utilizes state-of-the-art technologies and methodologies for chassis strain development, including genome editing tools, high-throughput screening techniques, and computational modeling. These advanced approaches enable efficient strain engineering and accelerated development timelines.
- CD BioGlyco fosters collaborative partnerships with clients, academia, and industry stakeholders to drive innovation and address complex challenges in glycan science and biotechnology.
CD BioGlyco stands out in Chassis Development due to its expertise, customized solutions, advanced technologies, quality assurance measures, comprehensive services, collaborative approach, and cost-effective solutions. Our capabilities empower clients to unlock the full potential of glycan-related applications and accelerate innovation in biotechnology and glycoscience. Please feel free to contact us for more information!
Reference
- Liu, J.; et al. Chassis engineering for microbial production of chemicals: from natural microbes to synthetic organisms. Current Opinion in Biotechnology. 2020, 66: 105-112.
For research use only. Not intended for any clinical use.
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