Client Satisfaction Is Our Cell-free System Construction Service Objective

CD BioGlyco constructs a variety of Chassis Cells to provide the possibility of efficient carbohydrate Production. In addition to Fungi, Bacteria, and Mammals, we also explore and develop other standard chassis with as many different features as possible to cover the largest range of applications. With rapid design-build-test cycles, high conversion rates, and open environments free from toxic proteins and metabolic mass transfer limitations, cell-free systems are exploited to address the multiple challenging technologies of early synthetic biology. We offer various cell-free system construction services for the synthesis of multiple sugars to support our client's Chassis Development and research needs.

The two cell-free system construction methods we commonly use are nucleic acid template-based and non-nucleic acid template-based methods.

• Construction of nucleic acid-based cell-free system

  We use two methods for nucleic acid-based cell-free systems. One approach is to artificially add various nucleotides, energy, RNA polymerases, cofactors, ribosomes, aminoacyl tRNAs, and templates for transcription and translation processes so as to mimic the intracellular environment in vitro. Various proteins required are eventually synthesized by executing the central law in vitro. Another method is to make a reaction system by extracting crude extracts from cells. The intracellular cofactors, various coenzymes, and energy-producing substances are acquired and maintained for their normal functions. The addition of nucleic acid templates to these acquired substances leads to the production of target proteins. We also add the appropriate substrates to catalyze the synthesis of the corresponding compounds from the proteins.

• Construction of cell-free systems based on non-nucleic acid templates

  For non-nucleic acid templates, we commonly use purified enzyme systems and crude lysis systems. We are well-versed in various cellular studies and specialize in constructing various carbohydrate synthesis pathways in vitro. The following cell-free systems are constructed.

Applications

• Cell-free systems are an important toolkit for synthetic biology and biotechnology research, providing new insights into understanding cellular processes outside the shell.
• Cell-free systems are utilized in the production of bio-based chemicals, functional ingredients, glycoproteins, and more.
• Cell-free systems give new opportunities for the expansion of applied scientific research on glycoproteins with novel structures and functions.

Advantages of Us

• We have extensive experience in the construction of cell-free systems for synthetic biology.
• We use a variety of strategies to construct cell-free systems.
• We have great flexibility in constructing cell-free systems. Cell-free systems for synthesizing a wide range of carbohydrates are constructed.

Frequently Asked Questions

• What are the advantages of cell-free systems for synthetic biology?
  • No metabolic mass transfer limitations due to the absence of cell membranes and cell walls.
  • Avoidance of possible toxicity limitations caused by unnatural products and metabolic intermediates.
  • Easy control of reaction conditions.
  • Prevents contamination of the microbial environment.
  • Reaction conditions are open and simple, only the conditions for synthesis of the product need to be met, and no cell growth conditions or sterility requirements need to be considered.
• What cell-free systems can be used for in vitro protein expression systems?
  E. coli, wheat germ extract, or rabbit reticulocyte lysates are all suitable for the construction of in vitro protein expression systems. All relevant cell-free systems can be constructed.

CD BioGlyco is equipped with a team of specialized researchers to complete projects related to the construction of cell-free systems. With our rich experience in constructs, we excel in accomplishing various challenges. Please feel free to contact us for more details about cell-free systems.

• Plant Chassis for Synthetic Biology
• Bacteria Chassis for Synthetic Biology
• Microalgae Chassis for Synthetic Biology
• Insect Chassis for Synthetic Biology
• Fungal Chassis for Synthetic Biology
• Mammalian Chassis for Synthetic Biology
• N-linked 3'-Siallylactose-based Cell-free System Construction Service
• 6'-Siallylactose-based Cell-free System Construction Service
• αGal Epitope Synthesis-based Cell-free System Construction Service
• Pyruvylated Lactose-based Cell-free System Construction Service
• 2'-Fucosyllactose-based Cell-free System Construction Service
• 3-Fucosyllactose-based Cell-free System Construction Service
• Mono- or Di- Fucosylated Lactose-based Cell-free System Construction Service
• Mono- or Di-Fucosylated LacNAc-based Cell-free System Construction Service
• Sialylated Lactose-based Cell-free System Construction Service
• Sialylated LacNAc-based Cell-free System Construction Service