Harnessing Bacillus natto for Cutting-edge Solutions at CD BioGlyco

Bacillus natto is an aerobic bacterium, which can compete with harmful bacteria for oxygen uptake, making the intestinal tract close to an anaerobic environment and achieving the effect of inhibiting and killing harmful bacteria. Due to the clear physiological and biochemical characteristics of Bacillus natto, its relatively simple genetic manipulation, and its high secretion and expression ability, it can serve as an excellent Bacteria Chassis Cell and be transformed into a microbial cell factory for the production of target products such as drug precursors. CD BioGlyco provides high-quality Bacillus natto engineering services with best-in-class synthetic biology technology.

Modification of Bacillus natto chassis cells

• Global regulation of chassis cells

  We optimize chassis cells by adjusting global regulators in chassis cells to reduce the metabolic burden due to heterologous production.
  For example, we re-edit the metabolic regulatory network in the strain by knocking out major global transcriptional regulators that block carbon metabolism and selecting Bacillus natto mutants that can utilize a wide range of carbon sources.

• Multidimensional regulation of chassis cells

  We utilize a weak, knockdown approach of unwanted branches to construct a multi-locus, multi-dimensional co-regulatory strategy that enhances the production status of Bacillus natto and facilitates the persistent and efficient production of desired products by chassis cells.
  Among them, we provide clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 technology based on the application of incision enzymes and homology-directed repair (HDR) is an effective method to realize the modification of large fragments and multilocus regulation of the Bacillus natto genome. Also, this method is more efficient for promoter library creation and screening.

• Bacillus natto genome streamlining and optimization

  To achieve maximum production efficiency and obtain optimal chassis cells, a large-scale reduction of the genome of the model strain is a good optimization strategy. We improve the efficiency of chassis cell synthesis by knocking out non-essential genes in the heterologous synthesis pathway and the coupling step of Bacillus natto's metabolism.

  • Bottom-up genome streamlining: The assembly of the basic modules (e.g., genes coding for essential proteins) needed to make up a cell, constituting the smallest biological unit that sustains cell life and viability.

  • Top-down genome streamlining: Deletion of a large number of non-essential gene portions of the genome to simplify existing cells, leading to chassis cell genome downsizing by the direction of production of the target product.

Genetic manipulation technologies of Bacillus natto

Certain strain genetic manipulation technologies and methods are effective tools to enhance the optimization process of Bacillus natto chassis cells. At CD BioGlyco, we offer technologies and methods that include, but are not limited to:

Fig.1 Metabolic engineering of Bacillus natto. (CD BioGlyco)

Applications

• Bacillus natto engineering can be used to synthesize small molecule compounds, enzymes, and other protein products. Examples include riboflavin, surface active agents, ethylene glycol, phytase, xylanase, chondroitin, and others.
• Bacillus natto engineering can be used to facilitate research into the production of functional food and pharmaceutical products.
• Bacillus natto engineering accelerates the evolutionary efficiency of Chassis Cells, Develops better chassis cells, and optimizes the regulatory steps of industrial production.

Advantages

• We have the world's leading biological technology to complete every project of our clients with high efficiency and quality.
• We provide custom services according to the needs of our clients, and each experimental solution has gone through strict quality testing.
• We have a strong technology team that is familiar with the biological properties of bacterial chassis cells used in synthetic biology and provide high-quality bacterial engineering services to our clients.

CD BioGlyco provides efficient chassis cell optimization and design, as well as Production Services to clients worldwide. Our experimental protocols are developed by experts with many years of experience in the field, providing professional assistance to our clients' research projects. Please feel free to contact us if you are interested in our services.

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