Dunaliella salina Engineering Service
Client Satisfaction Is the Purpose of Our Dunaliella salina Engineering Service
CD BioGlyco has developed a variety of Chassis for synthetic biology, including Plant Chassis, Microalgae Chassis, Insect Chassis, etc. D. salina, as a type of microalgae, is widely used for the Production of a variety of recombinant glycoproteins, biofuels, etc. D. salina engineering is an important technology to effectively facilitate production. With our rich experience in Chassis Development, we provide a professional and high-quality D. salina engineering service to help clients genetically manipulate it.
- D. salina engineering
D. salina is a novel expression system that is fast-growing, easy to culture, easy to manipulate transgenically, inexpensive to produce, and has transcriptional and translational capabilities. We use a variety of genetic engineering techniques to enhance the ability of D. salina to produce many recombinant glycoproteins and high-value carbohydrate-related compounds. We consider the effects of a variety of factors such as DNA delivery methods, and gene expression control before introducing desired genes into the algal genome.
- D. salina engineering vector optimization
Vector optimization is an essential step. We provide high-quality plasmids through comprehensive optimization.
- D. salina nuclear transformation
We offer a variety of nuclear transformations for D. salina to efficiently express the target product.
- D. salina chloroplast transformation
We perform chloroplast transformation through a variety of DNA delivery methods, including the establishment of transformation methods, modification of recombinant products, transgene codon optimization, and the use of strategies to improve transgene expression.
- Our gene editing technologies related to D. salina engineering
- Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas: CRISPR/Cas9-mediated targeted knockout, targeted knock-in, gene overexpression, and gene suppression.
- RNA interference (RNAi): Suppression of gene expression by blocking the transcription or translation of specific genes.
- Transcription activator-like effector nuclease (TALAN): TALEN is used to more efficiently edit genomic regions that are difficult to edit.
- What do we deliver?
- Modified D. salina
- High-value products produced (including glycoproteins, antigens, etc.)
- Detailed lab reports
Fig.1 Flowchart of our D. salina engineering. (CD BioGlyco)
Publication Data
Technology: Chloroplast transformation
Journal: Physiologia Plantarum
IF: 5.081
Published: 2021
Results: This paper describes several techniques and tools that can be used to engineer D. salina, including chloroplast transformation methods, gene editing techniques, and others. Several chloroplast transformation methods have been used for D. salina, including electroporation, agrobacterium-mediated splicing, glass bead method, and particle bombardment. In addition to this, some commonly used methods to improve the yield of proteins and other proteins, including codon optimization and the development of strong promoters, have been introduced, providing some effective strategies for D. salina engineering.
Fig.2 Strategies and techniques commonly used in microbial engineering. (Vavitsas, et al., 2021)
Frequently Asked Questions
- What are the advantages of D. salina for synthetic biology?
D. salina has been developed as a novel expression system for synthetic biology due to its many advantages.
- It is extremely adaptable to the environment.
- It can be cultured easily, quickly, and inexpensively.
- It can transcribe and translate.
- It lacks a rigid cell wall and is easily transformed with exogenous genes and downstream product extraction.
- It has large, cup-shaped, single chloroplasts.
- It can accumulate a large number of valuable fine components.
- High-value compounds can be selectively extracted from D. salina.
- What are the advantages of TALEN for D. salina engineering?
- TALEN is easier to design.
- Gene editing is more flexible, allowing more efficient editing of hard-to-edit genomic regions.
- TALEN has fewer off-target effects and exhibits less toxicity in human cell lines.
Applications
- D. salina engineering is used to enhance the production of natural high-value carbohydrate-related products, gene expression, etc.
- D. salina engineering is used for the production of recombinant glycoproteins, antigens, antibodies, etc.
- D. salina engineering is used for screening and functional validation of genes related to various carbohydrate-related synthetic and metabolic pathways.
Why Choose Us?
- We use several strategies to improve the production of glycoproteins, etc., such as codon optimization, transformation-related genotype modifications, etc.
- We combine multiple bioinformatics tools to analyze and design relevant synthetic and metabolic genetic pathways.
- We provide our clients with full follow-up services from protocol design to D. salina engineering to ensure the scientific validity of their experiments.
CD BioGlyco has a team of specialized scientists to support D. salina engineering services. Please feel free to contact us for more information about D. salina engineering.
References
- Vavitsas, K.; et al. Doing synthetic biology with photosynthetic microorganisms. Physiologia Plantarum. 2021, 173(2): 624-638.
- From Wikipedia (https://upload.wikimedia.org/wikipedia/commons/8/8a/FleurDeSel.JPG)
For research use only. Not intended for any clinical use.
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