Yarrowia lipolytica Engineering Service
Yarrowia lipolytica-An Industrial Production Host
CD BioGlyco is a professional company providing GlycoChas™ Cells Generation services such as Plant Chassis, Microalgae Chassis, Insect Chassis, Bacteria Chassis, and Mammalian Chassis. Y. lipolytica is a safe and reliable dimorphic yeast and its physiological, metabolic, and genomic properties make it a superior host for metabolic engineering. In combination with genetic engineering, CD BioGlyco exploits it as a cell factory by optimizing internal pathways and introducing new ones. Y. lipolytica contains 7357 genes encoding 6472 proteins. Therefore, Y. lipolytica is an ideal and efficient Fungal Chassis. Moreover, our lab has rich experience in Chassis Development.
Your Trusted Y. lipolytica Engineering Partner
CD BioGlyco has developed a one-step integration of a multigene strategy in Y. lipolytica. Moreover, our researchers intervene in the synthesis of terminators and plasmid processes for increasing expression levels and hybridizing synthetic promoters. We utilize a variety of synthetic biology and molecular biology tools to provide our clients with a range of engineering services such as transformation, overexpression, knockout, and labeling cycles to enable the Production of new substances.
Promoter
Promoters play a key role in controlling gene expression. We provide novel synthetic heterotrimeric promoter services to clients. In Y. lipolytica, common endogenous promoters are FBA1, POX2, GPM1, TEF1, GPD, GPAT, YAT1, TDH1, EXP1, LEU2, and XPR2. We have optimized common promoters by increasing mRNA and protein expression levels.
Y. lipolytica Vector
To obtain high gene dosage, we provide integration vector development and construction services. We integrate Y. lipolytica expression/secretion vectors into the genome by a single crossover. By improving the efficiency of homologous recombination, we increase the frequency of transformation and promote gene regulation such as overexpression and deletion. We provide professional one-stop service including replicative plasmids, construction of genomic inserts, constitutive and inducible promoters, fluorescent tags, protein expression, and secretion vectors. Moreover, for metabolites, we provide sequencing services.
Publication
Technology: Fluorescence assay, Genetic transformation, Gene overexpression, Gas chromatograph (GC), Polymerase chain reaction (PCR)
Journal: Biotechnology for Biofuels
Published: 2016
IF: 7.67
Results: In this work, researchers constructed a strain engineering strategy centered on the overexpression of the diacylglycerol acyltransferase (DGA) gene. This strategy was used to design high lipid-producing Y. lipolytica by combinations of overexpression and deletion of genetic targets and target screening for endogenous and heterologous targets and target combination screening. The results of multiple studies showed that DGA1 overexpression increased lipid content in Y. lipolytica when overexpression or deletion of genes that promote lipid accumulation when combined with DGA1 overexpression. In addition, continuous culture was preferred over batch or replenishment batch fermentation.
Fig.1 Overexpression of DGA2 genes in Y. lipolytica. (Friedlander, et al., 2016)
Applications
- Genetically engineered strains of Y. lipolytica are commonly used to produce single-cell oils required by human lysosomal enzymes for the treatment of lysosomal storage diseases.
- Y. lipolytica engineering is a valuable candidate pathway for both industrial and environmental applications.
- Y. lipolytica engineering plays an important role in the environmental bioremediation of aromatic compounds, organic pollutants, and aliphatic compounds.
- The biomass can be used as a safe nutritional additive in human food supplements to maintain organism homeostasis.
Advantages of Us
- The mRNA level of hybrid promoters synthesized by our company is several hundred times higher than the core promoter.
- Our lab is equipped with advanced instruments to meet the diverse needs of our clients.
CD BioGlyco attaches great importance to the cycle time and quality of our services, and are committed to providing the most satisfactory solutions for our clients. Our operators all have rich backgrounds in synthetic biology. We are committed to promoting the development of our client projects. Please feel free to contact us.
Reference
- Friedlander, J.; et al. Engineering of a high lipid producing Yarrowia lipolytica strain. Biotechnology for biofuels. 2016, 9: 1-12.
For research use only. Not intended for any clinical use.
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