Your Best Helper for Chlorella Chassis Cell Engineering Modification

CD BioGlyco has built the world-class GlycoChas™ Cells platform to provide engineering modification services for various types of chassis cells to clients around the world. We also provide Chassis Development services to our clients, including:

Chlorella is a single-cell eukaryotic algae with significant advantages such as high nutritional value, fast growth rate, simple structure, and easy industrial integration. Chlorella uses light energy and carbon dioxide for normal autotrophic growth under autotrophic conditions or uses organic carbon sources for growth and reproduction under heterotrophic culture conditions. Chlorella has many advantages as a host for exogenous gene transformation:

• It grows at high density under controllable conditions and accumulates a large amount of biologically active substances in a short period;
• When used as an expression system, high-level post-translational modifications are performed on proteins, and the post-translational modification mechanism is close to that of mammals, and can be used to produce useful substances such as recombinant vaccines and antibodies.

Therefore, scientists are constantly working hard to optimize the Chlorella transformation system and develop Chlorella transformation methods to continuously advance industrial Production and make it more widely used in biotechnology.

At CD BioGlyco, we have established a mature Chlorella exogenous gene transformation system. We introduce genes isolated from animals, plants, and microorganisms into Chlorella through various transformation technologies, turning it into a new and efficient bioreactor that is rapidly multiplied to produce various biologically active substances.

• Electroporation method: The electroporation method is a more commonly used transformation method in Chlorella transformation systems. This method uses external physical forces to open short-term micropores by applying a high-intensity electric field to cells under appropriate conditions and content, thereby achieving the purpose of transporting foreign genes into cells and performing gene integration. During the electroporation transformation process, factors such as buffer concentration, algae cell culture cycle, and DNA concentration all have an important impact on the transformation efficiency.
• Biolistic method: The biolistic method is a widely used and effective simple conversion method, suitable for almost all types of Chlorella such as hard cell walls. This method is to wrap the exogenous DNA in micron-sized tungsten powder or gold powder, and then accelerate the bombardment of the recipient cells through a particle bombardment gun in a vacuum state to achieve the transformation of the exogenous gene.

In addition, we also provide clients with the following services:

• Bioactive substance extraction service from Chlorella
• Bioactive substance purification service from Chlorella
• Characterization services of bioactive substances from Chlorella
• Activity determination of bioactive substances from Chlorella

Other Chassis Cells for Choosing

CD BioGlyco has accumulated rich knowledge and experience in the fields of genomics and molecular biology, using simple, specific, and efficient biosynthetic tools to modify and transform chassis cells to improve plant breeding and increase metabolite products. Chassis cells we handle include but are not limited to:

Plant Chassis

Microalgae Chassis

Insect Chassis

Fungal Chassis

Bacteria Chassis

Mammalian Chassis

Advantages of Us

• We help clients around the world modify and transform various types of chassis cells, such as plant chassis, microbial chassis, insect chassis, fungal chassis, bacterial chassis, and mammalian chassis.
• We have developed a mature Chlorella exogenous gene transformation system to help clients modify and transform Chlorella chassis cells to produce a variety of bioactive substances simply and efficiently.
• We not only help clients modify chassis cells to improve industrial production but also provide extraction, purification, and characterization services for biologically active substances.

Frequently Asked Questions

• How to optimize the expression effect of foreign genes in Chlorella?
  Study the genetic information of Chlorella species and the cloning and analysis of its functional genes, apply own genes to the research of other higher plants or microalgae, and promote the process of genetic transformation of Chlorella; Optimize the parameters of the existing transformation system and develop new Chlorella transformation methods to improve the expression effect of foreign genes in Chlorella; The transcription, translation, and post-translational modification mechanisms after foreign gene insertion, and whether the foreign protein is hydrolyzed have an important impact on efficient expression. An in-depth exploration of the molecular mechanism of the transformation process of Chlorella will help clarify the expression modification of foreign genes in Chlorella.
• What issues should be considered when cultivating microalgae?
  Select an appropriate microalgae culture technology system. Currently, two major types of microalgae culture technology systems have been formed, namely open pools and various types of closed photobioreactors; Control the light source, water source, and carbon source for the photosynthetic growth of microalgae; Solve the problem of harmful pollution in microalgae cultivation, there are three main modes of harmful pollution, namely protozoa pollution, bacterial pollution, miscellaneous algae and virus infection.

At CD BioGlyco, we have developed a variety of mature bioreactors to help clients produce target bioactive substances on a large scale. If you are interested in our services, please feel free to contact us.

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