Pioneering Progress: Pichia Pastoris Engineering Service at CD BioGlyco

Pichia pastoris, with its advantages of high growth density, strong protein expression, good genetic stability, and mild glycosylation modification, has shown excellent application in both academic research and industrial production and has become the preferred Fungal Chassis cell for recombinant protein expression. With deep knowledge of synthetic biology and first-class biology technology, CD BioGlyco has constructed Chassis Cell Platforms for a wide range of applications and provides Pichia pastoris engineering services to clients all over the world and applying them to the Production process of glycobiomolecules.

• Homologous Reorganization

  To obtain efficiently Modified Chassis Strains, CD BioGlyco provides clients with precise and efficient gene editing techniques for homologous recombination of Pichia pastoris.

  For example, knockdown of Ku70, a key gene for non-homologous end joining (NHEJ), overexpression of genes such as Rad52, which promotes homologous recombination (HR), and knockdown of the HR repressor gene Mph1. Among them, gene editing of Pichia pastoris using Ku70-deficient strains is also a common method for Pichia pastoris transformation.

• Application of Genetic Elements

  Pichia pastoris, as a non-model yeast, is modified by CD BioGlyco using specifically expressed and modified genetic elements. Examples include promoters, screening markers based on nutrient-deficient genes and resistance genes, and methanol-induced, glycerol-induced, and glucose-induced induction.

• Modification of Metabolic Pathways

  To increase the production capacity of Pichia pastoris cell engineering, CD BioGlyco offers Pichia pastoris metabolic pathway modification services.

• Introduction of heterologous enzymes to optimize the synthesis of natural metabolites
  We modify the Pichia pastoris metabolic pathway by introducing exogenous genes into the Pichia pastoris cell via specific plasmids. For example, we enhance xylitol production by expressing heterologous D-arabinitol and xylitol dehydrogenase.

• Integral knock-in of multiple heterologous genes to introduce new metabolic pathways
  We use multi-gene one-step editing technology to realize the one-step import of key genes for target products. For example, we achieve efficient synthesis of alkaloids in Pichia pastoris by introducing dozens of exogenous genes.

Fig.1 Bioengineering of Pichia pastoris. (CD BioGlyco)

Frequently Asked Questions

• What are the applications of Pichia pastoris engineering?
  As the simplest eukaryotic expression system, the Pichia pastoris system is endotoxin-free and has a richer post-translational modification mechanism for protein production than the prokaryotic expression system. Compared with insect and mammalian cell expression systems, it is faster and cheaper to produce. Currently, we have realized the efficient production of various antigens, industrial enzymes, and other protein products in Pichia pastoris.
  In addition, metabolic engineering modification or optimization of fermentation conditions in Pichia pastoris has enabled the production of a wide range of metabolites including, but not limited to, lactic acid, pyruvic acid, ethanol, polyketides, and terpenoids.
• What precautions should be taken in Pichia pastoris engineering applications?
  Pichia pastoris, as a methanol-nutrient yeast, suffers from low methanol utilization and sensitivity to methanol concentration. Therefore, when Pichia pastoris cell is engineered to produce some low-value-added chemical products, the methanol utilization rate will greatly affect the economy of production. Therefore, further metabolic engineering optimization of Pichia pastoris cells is needed to obtain better chassis strains for the production of methanol-related substances.

Advantages

• We have efficient Pichia pastoris engineering to provide custom experimental solutions to our clients.
• We have an experienced synthetic biology technical team to ensure that our client's research needs are met.
• We have a reliable and stable process to provide one-stop service and 24-hour after-sales service.

CD BioGlyco customizes detailed and rigorous experimental procedures according to the scientific needs of our clients, and our various advanced technologies can fully meet their requirements. Please feel free to contact us if you would like to learn more about our services in fungal chassis cell engineering.

• Saccharomyces cerevisiae Engineering Service
• Candida lipolytica Engineering Service
• Schizosaccharomyces pombe Engineering Service
• Kluyveromyces Engineering Service
• Yarrowia lipolytica Engineering Service
• Hansenula polymorpha Engineering Service
• Aspergillus Engineering Service
• Penicillium Engineering Service
• Trichoderma Engineering Service