E. coli Engineering Service
Strive to Create High-quality Escherichia coli Engineering Services for Our Clients
CD BioGlyco develops a variety of Chassis Cells for synthetic biology, including Plant Chassis, Fungal Chassis, Bacterial Chassis, etc. E. coli is a well-studied model bacterium that has been adapted for the Production of various carbohydrates, along with Campylobacter jejuni, Bacillus natto, Corynebacterium glutamicum, and others. Based on our extensive experience in bacterial Chassis Development, we provide a one-stop E. coli engineering service to fulfill our clients' various research needs related to synthetic biology. Our goal is to develop E. coli with a fully functional simplified genome and metabolic networks for more efficient synthesis of desired products.
- E. coli engineering
We identify indispensable genes in E. coli by combining experimental data, computational systems analysis, and modeling. Then, we modify and construct E. coli chassis by deleting non-essential genes using various strategies. After analyzing the biosynthetic pathways and genomes in E. coli as per the client's requirements, we design suitable biosynthetic pathways and introduce them into E. coli for the synthesis of various sugars.
- Strategies employed
We use top-down strategies (genome reduction) or bottom-up strategies (genome synthesis) to manipulate E. coli metabolism, including transferring genes, regulating gene expression, engineering proteins, etc.
Top-down strategy: Removal of non-essential genes to generate synthetic chassis.
Bottom-up strategy: Self-assembly from scratch into artificial chassis (based on the computer-designed genome sequence, design, synthesis, and assembly).
Fig.1 Flowchart of our E. coli engineering. (CD BioGlyco)
Publication Data
Technology: Top-down approach, bottom-up approach
Journal: Synthetic and Systems Biotechnology
Published: 2019
Results: This paper details two strategies (top-down strategy and bottom-up strategy) and some gene editing methods that can be used to engineer E. coli. This paper also lists some properties of E. coli chassis constructed by the top-down strategy to demonstrate the possibility of these two strategies, such as the ability to heterologously express the desired product, genome stability, and normal growth. This paper provides two very effective strategies and gene editing methods for E. coli engineering.
Fig.2 Retrofitting the microbial chassis using a top-down strategy. (Chi, et al., 2019)
Frequently Asked Questions
- What are the advantages of E. coli for synthetic biology?
With its well-defined genetic background, ease of handling, and cultivation, E. coli is one of the important hosts for engineered chassis. In addition to this, the availability of genetic tools makes it popular for chassis cell construction. A variety of complex pathways have been programmed into E. coli to give them complex functions, which provides a variety of data to support the utilization of E. coli.
- What technologies are available for E. coli engineering?
- Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas-assisted multiplexed automated genome engineering, λ Red recombination system, etc., are developed for editing the E. coli genome.
- DNA synthesis and assembly technologies (Gibson assembly, Golden Gate assembly, DNA assembly) enable bottom-up construction of E. coli.
- Various bioinformatics and computational tools are used to aid in E. coli synthetic pathway design.
Applications
- E. coli chassis constructed through E. coli engineering are used for healthcare and the production of renewable energy.
- E. coli chassis constructed through E. coli engineering are used for the production of various carbohydrate-related biomaterials.
- E. coli engineering is used to explore the structure and function of various genomes.
Highlights of Us
- Our familiarity with various carbohydrate-related synthetic and metabolic pathways guarantees us rational modifications of E. coli.
- The combined use of computational systems analysis, experimental data, and modeling helps us to reveal genes that are integral to the life of E. coli.
- We have a large chassis knowledge base to facilitate the development of accurate computer models.
CD BioGlyco is constantly updating and optimizing its synthetic biology technology to improve chassis construction. Welcome to contact us for more information about E. coli Engineering. Our researchers will be the first to solve any of your problems.
Reference
- Chi, H.; et al. Engineering and modification of microbial chassis for systems and synthetic biology. Synthetic and Systems Biotechnology. 2019, 4(1): 25-33.
For research use only. Not intended for any clinical use.
Related Services
