Sincerity and Honesty in HEK293 Glycoengineering Service at CD BioGlyco

CD BioGlyco has rich experience in gene editing technology and cell modification technology. We provide many types of Chassis cells for synthetic biology, including Plant Chassis, Fungal Chassis, Mammalian Chassis, etc. HEK293 cells are similar to the Chinese Hamster Ovary, which is a commonly used mammalian cell to express glycoproteins, Carbohydrate Antigens, etc. HEK293 glycoengineering plays an important role in gene function research, synthetic biology, and so on. Based on our rich experience in Chassis Development, we provide a one-stop HEK293 glycoengineering service from experimental design, vector construction, and transfection to mutant validation.

HEK293 glycoengineering

We design and construct synthetic pathways that lead to the stable Production of various recombinant glycoproteins, recombinant antibodies, and other products from HEK293 cells.

• Design: We design genetic pathways in HEK293 cells by combining bioinformatics and computational systems analysis tools.

• Modification: Based on various genome editing tools, we precisely edit the HEK293 genome by insertion, knockout, and other editing means.

• Validation of HEK293 mutants.

The gene editing technology we use

• Transcription activator-like effector nucleases (TALAN) gene editing technique

• Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 gene editing technology

• Multiple gene editing

What do we deliver?

• Modified HEK293 cell line

• Related glycoproteins, glycans, or other products

• Detailed experimental records and reports

Fig.1 Flowchart of our HEK293 glycoengineering. (CD BioGlyco)

Frequently Asked Questions

• What are the advantages of HEK293 cells for synthetic biology?
  Engineering HEK293 cells using gene editing technology to obtain stable cell lines suitable for various studies has become an important research material in modern biology. As one of the commonly used cells for eukaryotic protein expression, HEK293 cells have the following advantages:
  
  • Faster growth rate
  • Higher growth density
  • Higher transfection efficiency
  • Production of glycoproteins that more readily approximate the structure of human glycoproteins
• What to look for during plasmid transfection in HEK293 glycoengineering?
  • DNA purity: Endotoxin removal of DNA is also required when preparing high-purity DNA.
  • Cell density: Generally speaking, high transfection efficiency is achieved when the cell density reaches 70% to 90% for transfection. Too low or too high will affect the transfection effect.
  • Cell type: Different cell types require different transfection systems for transfection.
  • Cell status: To improve transfection efficiency as well as transfection stability and reduce cytotoxicity, we try to use moderately passaged cell lines and keep the number of cell passages consistent in different experiments.
  • Transfection reagents: The principles of transfection reagents are high efficiency, low toxicity, convenience, and inexpensive. It is important to select the transfection reagent that best suits the experimental design.

Applications

• HEK293 glycoengineering is used to validate various carbohydrate-related gene functions, construct disease models, and study gene therapy approaches.
• HEK293 glycoengineering is used in studies such as genome-wide functional screening.
• HEK293 glycoengineering is used for the production of various glycoproteins, glycans, and other carbohydrates.
• Manipulation of glycosyl decoration or cell surface glycosylation of selected proteins by HEK293 glycoengineering has great potential for applications in improving glycoprotein quality or exploring structure-activity relationships.

Advantages of Us

• We are proficient in a variety of gene editing techniques. We effectively improve the success rate of editing by selecting the appropriate gene editing technique for the specific purpose.
• Our experienced technical team develops the most appropriate and scientific experimental design that meets the client's requirements.
• We significantly increase the yield of target product expression through vector sequences (codon optimization of expressed gene sequences, low molecular weight vectors, signal peptides with strong secretion effects, etc.), transfection methods, and cell cultures.

CD BioGlyco adheres to a high degree of scientific rigor and strict quality control to provide excellent HEK293 glycoengineering services for major research colleges, research institutes, and enterprises. Welcome to contact us for more information about HEK293 glycoengineering.

• Chinese Hamster Ovary (CHO) Engineering Service