Phaeodactylum tricornutum Engineering Service
Designing a Sustainable Future with Phaeodactylum tricornutum Engineering Services
Phaeodactylum tricornutum, as a diatom model organism, is characterized by fast reproduction and easy regulation. Therefore, Phaeodactylum tricornutum is expected to be a good Microalgae Chassis for use in synthetic biology. At CD BioGlyco, we utilize biological methods, combined with gene editing multiple technologies, to provide our clients with an efficient Phaeodactylum tricornutum engineering service and for the Production of glyco-biomolecules.
High-quality Bioengineering with Phaeodactylum tricornutum at CD BioGlyco
Phaeodactylum tricornutum Engineering Vector Optimization
We provide different types of plasmid modules to our clients for Phaeodactylum tricornutum engineering, and we also provide optimized plasmid vector services to improve transformation efficiency.
Screening for endogenous promoters
We screen for promoters with excellent performance to enhance the expression of exogenous genes. At the same time, we utilize the "promoter capture method" to select suitable endogenous promoters, and then randomly integrate selective marker genes around the strong promoters.
Optimizing codons
The lack of tRNAs used with specific gene codons can affect gene expression. We provide our clients with high-quality codon optimization services to promote exogenous gene expression.
Gene Editing
Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system
Targeted knock-out: We acquire specific genes through the sgRNA-mediated CRISPR-Cas9 system.
Targeted knock-in: Our combined use of particle bombardment transformation technology allows targeted insertion of donor DNA into the Phaeodactylum tricornutum genome.
Gene overexpression/repression: we utilize the CRISPR-Cas9 system to upregulate Phaeodactylum tricornutum target genes. Meanwhile, it can be used to repress Phaeodactylum tricornutum target gene expression after combining sgRNA and dead Cas9 to transform stable red fluorescent protein-expressing cells.
In addition, we carry out cell line screening and genotyping strategies using a combination of manual sequencing and tracking indels by deconvolution (TIDE) sequencing analyses to screen for Phaeodactylum tricornutum mutant cell lines.
Transcription activator-like effector nuclease (TALEN) technology
Multi-gene editing: We offer a wide range of gene editing services according to homology-directed repair and non-homologous end joining based on TALEN technology.
- Knock-in
- Creation of stop codons
- Suppression of coding RNA
- Deletion of chromosome segments
Publication Data
Technology: CRISPR-Cas9 technology
Journal: Frontiers in Plant Science
IF: 5.6
Published: 2018
Results: In this article, the authors constructed a vector with efficient expression based on the CRISPR-Cas9 system and used it for Phaeodactylum tricornutum.Then, the authors tested the function of the vector. In their experiments, the authors targeted several different genes, screened Phaeodactylum tricornutum for mutations in multiple steps, and analyzed the performance of Phaeodactylum tricornutum after the mutations. In addition, the authors analyzed cells showing allele-targeting mutations. Finally, the authors determined that the application of the CRISPR-Cas9 system in Phaeodactylum tricornutum is both efficient and widespread.
Applications
- Phaeodactylum tricornutum engineering can be used in the development of nutritional and cosmetic products as microalgae are widely used as raw materials for the production of nutritional and cosmetic products.
- Microalgae have the potential to serve as chassis cells for the production of many high-value compounds and proteins, so Phaeodactylum tricornutum engineering can be used to enhance the production of high-performing recombinant proteins, including antibodies, hormones, vaccines, and insecticidal proteins.
- The use of genetically engineered Phaeodactylum tricornutum enables more time- and labor-efficient production of a wide range of target substances.
Advantages
- With several years of research experience in Chassis Development, we provide professional and high-quality microalgae engineering services to our clients to help them genetically manipulate microalgae.
- We utilize microalgae engineering to produce target biomass at scale in a short time.
- We have a team of specialized Chassis Cell research scientists to support any of our clients' research needs.
In the field of algae engineering research, CD BioGlyco has accumulated proven and efficient technologies to provide first-class services to our clients. We utilize synthetic biology, automation, and other technologies to form synergistic collaborations with databases to efficiently meet our clients' research needs. Please contact us for more information.
Reference
- Stukenberg, D.; et al. Optimizing CRISPR/Cas9 for the diatom Phaeodactylum tricornutum. Frontiers in Plant Science. 2018, 9.
For research use only. Not intended for any clinical use.
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