Excellent Team, High-quality Mammalian Chassis

CD BioGlyco enables Plants, Microalgae, Bacteria, and mammalian cells by introducing new features into them that allow them to increase the production of metabolites or produce something that would not be produced in the natural state. Based on our extensive experience in Chassis Cell Development, we offer a high-quality mammalian chassis development service for synthetic biology. Our goal is to create new biological functions by designing and controlling the assembly of mammalian genetic circuits.

Mammalian cells are excellent hosts for the Production of many high-value-added products such as glycoproteins. We develop a set of precise and scalable tools (high-throughput analysis of DNA regulatory elements, targetable DNA binding systems, etc.) to characterize and control carbohydrate-related gene expression and function for sugar-related synthetic biology work in mammalian cells. We systematically profile and quantify the function of different DNA regulatory modules (e.g. promoters and enhancers) in mammals with high throughput and sensitivity, providing genetic components for customizable gene circuit construction related to glycoproteins.

We have a variety of gene editing techniques for Chassis construction. The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system is the basis for this development of tools that provide programmable and site-specific control over mammalian chassis genes. This precise control enables us to construct multilayered gene circuits with higher-order functions in mammalian cells, leading to the efficient production of a wide range of glycoconjugates. In addition to this, we introduce specific carbohydrate-related synthetic pathways into mammalian cells or enhance the production of high-value metabolites such as glycoproteins.

Fig.1 Flowchart for the construction of mammalian chassis for synthetic biology-related research. (CD BioGlyco)

We offer the following two main types of highly efficient cell lines for carbohydrate production. Each cell line engineering program is divided into several phases. At each stage, we maintain close technical communication with our clients so that the project progresses smoothly.

Frequently Asked Questions

• What are the advantages of CHO cells in synthetic biology research?
  CHO cell offers several advantages as a mammalian cell line expression system. It is well tolerant to changes in parameters such as oxygen levels, temperature, pH, or cell density, making them ideal host cells for large-scale cultures. Most importantly, it is capable of producing proteins with complex glycosylated, post-translational modifications (PTM). These proteins are similar to those produced by humans and these cannot be realized in bacteria, for example.
• What are the advantages of HEK293 cells in synthetic biology research?
  HEK293 cells are versatile cell factories suitable for a wide range of product types. Its ability to generate human glycosylation patterns and its better ability to process functionally essential post-translational modifications make it an ideal chassis cell for use in the manufacture of specific secreted biopharmaceuticals.

Applications

• Mammalian chassis for synthetic biology add new tools for modifying pathways associated with glycoprotein synthesis and more.
• Mammalian chassis for synthetic biology offers a wide range of strategies for gene- and cell-based carbohydrate production research.
• Mammalian chassis for synthetic biology are used to produce carbohydrate antigens and more.

Advantages of Us

• We have accumulated years of expertise in CHO and HEK293 genome editing.
• We have developed gene editing tools that help reduce the development time of cell lines.
• Throughout the mammalian chassis development process, our R&D team maintains close technical communication with our clients so that the project progresses smoothly.

CD BioGlyco draws on years of expertise in genome editing of CHO and HEK293 cell lines to support our clients' biomanufacturing and research. Our customized services use a variety of molecular biology methods with guaranteed quality. Please feel free to contact us for more information on mammalian chassis cells for synthetic biology.

• Plant Chassis for Synthetic Biology
• Bacteria Chassis for Synthetic Biology
• Cell-free System Construction Service
• Microalgae Chassis for Synthetic Biology
• Insect Chassis for Synthetic Biology
• Fungal Chassis for Synthetic Biology
• Chinese Hamster Ovary (CHO) Engineering Service
• HEK293 Glycoengineering Service