High-quality Fungal Chassis to Win Clients' Trust

CD BioGlyco constructs a variety of Chassis Cells for synthetic biology through computational design, synthetic re-engineering, and editorial regulation. The chassis cells we construct include Plant Cells, Insect Cells, Bacterial Cells, fungal cells, etc. Fungi have various advantages and are used to synthesize various sugars after modification. We provide a fungal chassis cell construction service for synthetic biology and various fungal chassis cells for a variety of sugars to support and satisfy our clients' synthetic biology-related research needs. Based on our extensive experience in Chassis Cell Development, we focus on modification by introducing desired traits into fungal chassis cells or enhancing the synthesis of high-value metabolites. Our goal is to construct fungal chassis cells that are safe, efficiently utilize a variety of substrates, have a short growth cycle, a high yield of target products, few by-products, and stable performance.

Chassis cells are the basis for designing and constructing artificial biological cells in synthetic biology. By placing functionalized biological system modules in fungal chassis cells, we enable them to have the specific functions we need, such as heterologous expression of various sugars.

• Our construction strategies are as follows:
  • We use two strategies, bottom-up gene assembly and top-down genome streamlining, alone or in a complementary fashion.
  • We perform genome editing, metabolic pathway reconstruction, heterologous host expression, and gene knockdown via clustered regularly interspaced short palindromic repeats (CRISPR)-Cas technology.
  • We express multiple gRNAs in tandem to rapidly achieve simultaneous multi-gene modification of the genome and yield enhancement.
  • We combine genetic engineering, mutagenesis, experimental evolution, and computational modeling to solve problems encountered in fungal chassis cell development.
• The steps of fungal chassis cell construction are as follows:
  • Design: Rational design of fungal genes, metabolic pathways, or genomes with existing standardized biological components.
  • Construction: Including DNA synthesis, large fragment assembly, and gene editing.
  • Mutant confirmation: Detecting whether fungal chassis cells are successfully constructed.

We enhance the sugar production capacity of various fungal chassis cells by modifying their metabolic networks. The fungal chassis cells we use for synthetic biology include the following:

In addition to the fungal chassis cells mentioned above, we also offer a variety of Carbohydrate Products.

Frequently Asked Questions

• What are the advantages of fungal chassis cells for synthetic biology?
  Fungi have the advantages of simple genetic manipulation, fast growth, clear background, easy to culture on a large scale, and relatively mature industrial fermentation culture mode. At the same time, fungi are able to enhance the production of original products by artificially designing regulatory elements and optimizing the original metabolic network system or by synthesizing natural products or even non-natural products with novel structures by rebuilding new metabolic networks.
• What are some of the techniques used to construct fungal chassis cells for synthetic biology?
  Bioinformatics or metabolic network modeling is used to identify essential and non-essential genes by comparing them with existing databases of essential and non-essential genes. The identification of essential and non-essential genes is also often combined with experimental methods such as RNA interference and transposon mutation library methods. In the process of deletion of non-essential genes, genome editing and large fragment knockout technologies play a key role, and commonly used technologies include CRISPR-Cas system-mediated gene knockout methods, Cre-loxP recombination system, FLP-FRT system, and λ-Red homologous recombination system.

Applications

• Fungal chassis cells are used in food, medicine, and chemicals.
• Fungal chassis cells are used for heterologous expression of natural products and their derivatives.
• Fungal chassis cells are used for the production of various potential drugs.

Advantages of Us

• During the development of our fungal chassis cells, we apply a variety of analytical tools (predictive analysis of promoters, terminators, and manipulators) and perform codon optimization based on the codon preferences of the selected chassis cells using relevant software programs.
• During the development process, we consider the effects of various factors on fungal chassis cells, such as fermentation type, fermentation vessel, and medium.
• We summarize a set of synthetic biology and computational tools for the construction and optimization of fungal chassis cells.

CD BioGlyco constructs a variety of chassis cells to aid in synthetic biology research. Please feel free to contact us for more details about fungal chassis cells. Our professional research team will get back to you as soon as possible.

• Plant Chassis for Synthetic Biology
• Bacteria Chassis for Synthetic Biology
• Cell-free System Construction Service
• Microalgae Chassis for Synthetic Biology
• Insect Chassis for Synthetic Biology
• Mammalian Chassis for Synthetic Biology
• Saccharomyces cerevisiae Engineering Service
• Pichia pastoris Engineering Service
• Candida lipolytica Engineering Service
• Schizosaccharomyces pombe Engineering Service
• Kluyveromyces Engineering Service
• Yarrowia lipolytica Engineering Service
• Hansenula polymorpha Engineering Service
• Aspergillus Engineering Service
• Penicillium Engineering Service
• Trichoderma Engineering Service