Halomonas Engineering Service
Revolutionizing Solutions with Halomonas Engineering Excellence at CD BioGlyco
Halomonas is a class of moderately halophilic microorganisms that can grow healthily at salt concentrations ranging from 5% to 25%. Based on good salt tolerance, Halomonas has great potential for development in the field of biology. At CD BioGlyco, we utilize synthetic biology technology to scientifically modify Halomonas chassis cells, and provide high-quality Halomonas engineering services to help our clients obtain good engineering strains that can meet the needs of production and life.
- Modification of Chassis Cells
We offer chromosomal recombination, clustered regularly interspaced short palindromic repeats (CRISPR), and the CRISPR-Cas9 system consisting of CRISPR and the related protein Cas to transform Halomonas Bacteria Chassis.
Among them, the CRISPR-Cas9 system can either inhibit the transcription of target genes by directing Cas proteins to form a spatial block through targeted DNA sequences or edit genes (knockout, insertion, and substitution of target sites) through targeted DNA sequences in conjunction with Cas proteins.
- Regulation of Halomonas
We apply metabolic engineering in synthetic biology (static and dynamic regulation) to modify the metabolism of Halomonas chassis cells.
Static regulation: We construct Halomonas strains by gene deletion and insertion, gene expression level regulation, ribosome binding site modification, and metabolic flow balance analysis. We also provide models to predict the changes in gene expression levels and metabolism after gene knockdown.
Dynamic regulation: We regulate the metabolic regulation of Halomonas chassis cells triggered by biosensors sensing signaling molecules with the regulation of target products as a result.
Publication Data
Technology: CRISPRi system
Journal: Microbial Cell Factories
IF: 6.4
Published: 2017
Results: In this research, the authors successfully applied CRISPRi to Halomonas. Experimental results showed that a non-model microorganism Halomonas species TD01 inhibited the expression of the ftsZ gene encoding the bacterial fission ring formation protein, causing an elongated cellular morphology. CRISPRi was used to regulate the expression of the gene encoding the prpC gene expression, and effective regulation of the 3-hydroxyvalerate monomer ratio in copolymers of 3-hydroxybutyrate and 3-hydroxyvalerate could be achieved. Moreover, TD01, whose gltA gene was inhibited by CRISPRi at different intensities, showed an increase of about 8% compared with the wild-type control with the untargeted CRISPRi vector.
Applications
- Halomonas enables continuous open fermentation using seawater, so Halomonas engineering helps salt-tolerant bacteria produce cumulative industrial products in seawater media.
- Because Halomonas can grow rapidly in extreme environments with high salinity and high pH, Halomonas engineering helps to construct cell factories for continuous fermentation and process perfection.
- Halomonas engineering can be used to produce a wide range of bio-based premium materials with different properties, such as polyhydroxy fatty acid esters (PHA).
Advantages
- We have high-efficiency and high-quality Chassis Cell Development Services.
- We pay attention to clients' feedback on time and provide comprehensive, professional after-sales service.
- We provide custom experimental solutions according to client's needs.
CD BioGlyco provides clients with best-in-class synthetic biology services, such as chassis cell construction and the Production of glyco-biomolecules. Our experts have exceptional expertise and skills to ensure that our clients' scientific needs are met. Our services have been recognized and praised by all our clients. Please feel free to contact us at any time for more information.
Reference
- Tao, W.; et al. Engineering Halomonas species TD01 for enhanced polyhydroxyalkanoates synthesis via CRISPRi. Microb Cell Fact. 2017, 16: 48.
For research use only. Not intended for any clinical use.
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