Trichoderma contains a wide range of rhizosphere soil fungi with parasitic and decaying properties. It is widespread in marine and terrestrial ecosystems. Trichoderma is an indispensable Fungal Chassis with strong maneuverability for large-scale production of high-quality Products. CD BioGlyco provides Trichoderma-engineered strains through genome-wide gene disruption, gene replacement, precision editing, and modulating their intrinsic genetic networks. Our professional and flawless GlycoChas™ Cells and Chassis Development solutions drive our customers' synthetic biology projects. In addition, we provide the following different chassis for synthetic biology:

Empower Product Quality with High-level Trichoderma Engineering

Strains and culture condition

CD BioGlyco has various high-expression Trichoderma fungi, including T. reesei, T. harzianum, T. virens, T. longibrachiatum, T. koningii, T. pseudokoningii, T. asperellum, T. atroviride and T. viride. Moreover, we provide culture condition optimization services by one-factor, two-factor, and response surface tests.

Gene regulation

CD BioGlyco provides comprehensive engineered strain construction services including, the design of plasmids with vector construction, transfection, electroporation, biolistic bombardment, strain modification, transformation, and functional gene validation services.

• Our researchers use clustered regularly interspaced short palindromic repeat sequences/CRISPR-associated nucleases 9 (CRISPR/Cas9) to modify different genomes by introducing double-strand breaks and activate sequence variation by non-homologous end joining (NHEJ). We offer simultaneous editing services at multiple loci to accomplish the introduction or deletion of genes quickly and accurately.
• With the aid of homology-directed repair, we provide precise sequence alterations.
• We provide high-efficiency conversion service by polyethylene glycol (PEG) -mediated protoplast transformation (PMT) and Agrobacterium tumefaciens-mediated transformation (APMT).
• We verify gene (cluster) function by DNA sequencing.

Fig.1 Schematic diagram of CRISPR-Cas9 systems in Trichoderma engineering service. (CD BioGlyco)

Applications

• Trichoderma engineering strain can be used to produce biofertilizers, biocides, and bioremediation agents.
• Due to the wide host range and adaptability of Trichoderma, it can be used for the production of multiple enzyme preparations e.g. cellobiohydrolases, endo-β-1,4-D-glucanases, and β-D-glucosidases.

Frequently Asked Questions

• How to enhance the secretion and expression of ideal products?
  For the Trichoderma engineering strain, the development of new promoters and expression systems effectively increased the yield of target substances. By replacing the transcriptional repressor (cre1, ace1) with transcriptional activators (xyr1, ace2/3, hap2/3/5), strong constitutive promoters are induced with the help of genetic engineering to recognize the integration and transform into hybrid promoters. Moreover, random mutagenesis is also a good strategy to improve the yield. Some more high-quality strategies include high expression of gene promoters, achieving position-independent expression, increasing the copy number of the gene of interest, reducing protein degradation, and improving the culture medium.
• What properties of promoters are expressed from high-quality homologous and heterologous proteins?
  • Promoters with higher expression intensity were achieved by increasing the copy number.
  • Small or no need for culture medium requirements.
  • Reduction or no expression of unrelated proteins.

Combined with synthetic biology tools, CD BioGlyco provides the construction and development of high-titer GlycoChas™ cells. Our lab has professional instruments and an advanced analysis system to promote the development of client genetic engineering projects. Please feel free to contact us for more information.

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