Bringing Natural Glycan Production to Perfection

Natural glycans, which are often synthesized by living organisms, are biomolecules located in the cell wall, intracellular, intercellular, and extracellular in the secretory plasma, and they are essential components of life activities. Natural glycans are an important part of the structure of living organisms, they can store energy, regulate and modify the structure and function of proteins, and also influence cellular interactions and information transfer. At CD BioGlyco, we provide comprehensive and efficient natural glycan production services with our specialized biological knowledge.

Harnessing Synthetic Biology for Natural Glycan Production at CD BioGlyco

Biological extraction

Most of the natural glycans can be found in plants, bacteria, or fungi, so extraction is a simple and convenient way to produce natural glycans. Enzymes are a powerful tool in the extraction of natural polysaccharides, and we need to screen for suitable enzymes to assist in the extraction of natural glycans under natural pH conditions.

We pulverize the raw materials in the presence of glycans and make a homogenate. Then, it is centrifuged and the supernatant is separated. Next, corresponding enzymes are added to the supernatant for the extraction of natural glycans. After the inactivation of the enzymes, the supernatant is dialyzed. Finally, freeze-drying is performed to obtain the corresponding natural glycans.

Microbial fermentation

Microbial fermentation strains contain fungi, bacteria, or some tiny algae. We offer solid-state fermentation, deep liquid fermentation, and bi-directional fermentation for the production of natural glycans. At the same time, the selection and breeding of strains can effectively realize the large-scale production of natural glycans. To achieve the yield of natural glycans, we optimize the medium composition and culture conditions.

• The composition of the medium contains a variety of carbon and nitrogen sources required for microbial growth. We utilize orthogonal tests to screen the optimal medium composition for microbial fermentation.

• In terms of culture conditions, such as temperature, pH, and time, affect the growth state of microorganisms. We still utilize orthogonal tests to screen the most suitable culture conditions for microbial growth.

Plant glycoengineering

Plant glycoengineering allows the preparation of natural plant glycan-based structures. A key step in plant glycoengineering is the preparation of plant chassis with uniform glycan-based structures. In this regard, we utilize genomic mutation knockout, gene editing, and gene silencing to eliminate genes encoding redundant glycan bases and glycosyltransferases, which in turn results in homogeneous plants and plant cell cultures.

For example, in the case of complex N-glycans, the GlcNAc2Man3GlcNAc2 (GnGn) structure is a suitable "Chassis", which is the smallest structure that can serve as a receptor substrate for further elongation and diversification steps. To generate a plant chassis that provides a homogeneous GnGn structure, we need to eliminate plant-specific residues (i.e., core xylose and fucose), ablate HEXO activity, and eliminate the Le^a structure.

Here, CD BioGlyco offers the production of natural glycans comprising, but not limited to, the following glycan/glycan structures:

Applications

• Most of the natural glycans are biocompatible and non-toxic and can be used in the development of food additives and emulsifiers.
• Natural glycans can be effectively used in the development of slow-release materials for drugs and gene transduction vectors due to their anti-cancer, lipid-lowering, and immune-enhancing physiological functions.
• Natural glycans can be used as fungicides, plant growth regulators, pesticide release agents, fruit and vegetable preservatives, and biodegradable membranes in agriculture due to their special properties such as bacteriostatic, crop growth regulator, biocompatibility, and biodegradability.

Advantages

• We provide a one-stop natural glycan production service to our clients. For example, production of natural glycans, structural characterization, and bioactivity analysis.
• With cutting-edge synthetic biology technology, we have constructed a specialized Chassis Cell Platform to provide tailor-made Production Services to ensure client satisfaction.
• Our production solutions are diversified, covering many types of glycan structures with high efficiency and quality.

CD BioGlyco has a top-notch glycan production team dedicated to the production of glycans of different structures and origins. Our natural glycan production technology can support your research in the field of glycobiology. If you are interested in our services, please feel free to contact us.

• HMO Production Service
• Carbohydrate Antigen Production Service
• Bioactive Ingredient Production Service
• Specific Glycan Molecule Production Service