Candida lipolytica Engineering Service
Candida lipolytica Engineering: CD BioGlyco's Engineering Excellence
Candida lipolytica, a non-traditional yeast, can promote cell growth through respiration and has many excellent physiological and metabolic properties. In addition, Candida lipolytica is an excellent strain for the production of single-cell proteins by petroleum fermentation. In particular, in the field of biology, Candida lipolytica engineering plays an important role in the Production of some glycobiomolecules. At CD BioGlyco, we construct turf-specific Chassis Cell platforms and provide Candida lipolytica engineering service to each of our clients with our cutting-edge synthetic biology technology.
Candida lipolytica Gene Engineering
Candida lipolytica expression system
Efficient expression vectors are a prerequisite for achieving exogenous gene expression in the host. Therefore, we provide important components such as suitable screening markers, safe and efficient promoters, and signal peptide sequences to construct expression vectors to ensure that excellent Candida lipolytica hosts are obtained.
For example, actinomycin ketone (CYH) can effectively inhibit protein synthesis in eukaryotes, and we use it as a screening marker to construct the wild Candida lipolytica expression system. At the same time, to provide clients with customized solutions, we offer a variety of promoters. These include PGK-pr (phosphoglycerate kinase), PMA-pr (plasma membrane ATPase), and ACT1-pr (actin), etc.
Candida lipolytica knockout system
We modify Candida lipolytica strains at the genetic level to carry out functional genomics research. We offer diverse gene knockout technologies. For example, Cre-loxP technology. In this technology, Cre recombinase can mediate specific recognition between two loxP sites so that the gene sequence between the loxP sites is knocked out or recombined.
Candida lipolytica genetic transformation
Efficient genetic transformation methods are the basis of gene engineering research on Candida lipolytica. Recombinant plasmid vectors are linearized by endonuclease, and the integration efficiency in yeast can be greatly improved. We provide Candida lipolytica transformation methods such as the protoplast method, electroshock method, polyethylene glycol method, and so on.
Candida lipolytica Metabolic Engineering
Broadening the spectrum of substrate utilization
We utilize the metabolic engineering of strains to broaden the substrate spectrum of production strains for the synthesis of various chemicals using different raw materials as carbon sources. We obtain recombinant Candida lipolytica strains with enzymatic activity by expressing specific enzymes (e.g. reductases, kinases, etc.) or genes encoding enzymes in the Candida lipolytica strain. This in turn leads to the production of chemicals in an environment with different carbon sources.
Enhancing the supply of precursor substances
An adequate supply of precursor substances is necessary to ensure a high yield of target products. Among many metabolites, acetyl coenzyme A is the central hub of anabolic and catabolic metabolism in organisms and an essential precursor for the synthesis of many chemicals. Therefore, we aim to improve the production capacity of Candida lipolytica by enhancing the supply of acetyl coenzyme A.
Publication Date
Technology: Gene knockout
Journal: Microbial Cell Factories
IF: 6.4
Published: 2021
Results: In this research, the authors designed a Candida lipolytica strain lacking uracil to efficiently convert xylose mother liquor (XML) to the commercially valuable product xylitol. During the experiment, the authors blocked the conversion of xylitol to xylulose by deleting the gene for xylitol dehydrogenase. The regeneration system was added by heterologous expression of the Candida lipolytica gene encoding gluconate dehydrogenase. After continuous exploration of the experimental process, the authors increased the yield of xylitol to 92.40%.
Fig.1 Process of xylitol production. (Zhang, et al., 2021)
Applications
- Candida lipolytica engineering can be used to develop diagnostic and therapeutic methods for diseases.
- Candida lipolytica engineering is used in a wide range of biotechnology development and industrial applications. Examples include the large-scale production of natural products, organic acids, and alcohols.
- Candida lipolytica is a non-traditional yeast and therefore Candida lipolytica engineering can be used to explore the use of non-traditional yeasts in the production of products.
Advantages
- We customize our Chassis Cell Development solutions to meet our clients' scientific needs.
- We have a systematic synthetic biology technology platform and a skilled production system.
- Our Fungal Chassis engineering is characterized by stability, high efficiency, and wide application.
- We provide one-stop service and 24-hour after-sales service to our clients.
CD BioGlyco provides first-class and attentive fungal engineering services with world-leading synthetic biology technology. Our service team is composed of experts in the field of biology to ensure that our client's requirements are met. We sincerely hope to be your right-hand man. Please feel free to contact us if you still have any questions, and we will answer you promptly.
Reference
- Zhang, L.; et al. Stepwise metabolic engineering of Candida tropicalis for efficient xylitol production from xylose mother liquor. Microb Cell Fact. 2021, 20: 105.
For research use only. Not intended for any clinical use.
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