Pseudomonas putida- A Robust Synthetic Biology Chassis

P. putida is a soil Bacterium generally recognized as safe (GRAS). It has a central carbon metabolism network that meets the high demand for reducing capacity. CD BioGlyco has a professional talent team to help clients build P. putida engineering chassis and develop new biological Products with a range of synthetic biology tools. Our staff helps clients select the most suitable host based on the project features. Our team has accumulated extensive experience in building GlycoChas™ Cells and Chassis Development to ensure efficient project delivery.

Empowering Excellence Through P. putida Engineering Service

Designed genome reduction optimizes cellular metabolic pathways and improves utilization, production, and physiological performance predictability to some extent. Analysis, design, modification, and recombinant expression of P. putida are necessary. CD BioGlyco provides a range of services:

Professional analysis

CD BioGlyco provides chromosome sequence analysis and metabolic pathway analysis of P. putida strains using state-of-the-art analysis systems. Moreover, we provide protein coding gene identification and metabolic pathway optimization services.

Design and construction

With the help of gene editing, CD BioGlyco provides precise P. putida genome deletion or introduction services. We use genomic islands in the P. putida genome as deletion targets to construct deletion strains and use scarless large genomic DNA fragment deletion to knock out genomic islands.

To ensure successful deletion of the target genomic region, we provide specific polymerase chain reaction (PCR) assay services and DNA sequencing of the amplified fragments. Moreover, we provide professional transcriptome analysis and imaging analysis.

Test and evaluate

To determine the optimal chassis, we provide physiological characterization services for translational capacity, heterologous protein expression, metabolic activity, production capacity, chromosomal integration capacity, and genetic stability.

Applications

• Biomanufacture: P. putida engineering has various transport and metabolic systems, enabling it can be used in biocatalysis, heterologous production, biodegradation, and bioplastics production.
• Environmental bioremediation agents: P. putida engineering strain plays an important role in environmental bioremediation of industrial waste.
• Bioengineering: P. putida engineering strain is often used in bioengineering for biosynthesis, protein expression, and other studies because of its high metabolic capacity and adaptability to various environmental conditions.

Frequently Asked Questions

• Why choose P. putida as a robust physical and metabolic chassis?
  • P. putida contains 1256 degradation reactions including a newly discovered catabolic pathway comprising 32 carbon, 29 phosphorus, 28 nitrogen, and 3 carbon and nitrogen sources. Moreover, the P. putida strain has a very unique core metabolic structure with the connectivity of sugar assimilation. In addition to the above, it retains the ability to grow and prosper rapidly in different environmental species. It remains stable and tolerant under adverse conditions of nutrient demand and oxidative stress. Thus, the P. putida strain is a good host for cloning and gene expression and is endowed with biomanufacturing capabilities.
• How is central carbon metabolism fueled by sugars in P. putida?
  • Many Pseudomonads contain not only the common phosphorylation pathway but also a specific peripheral pathway for sugar oxidation. Notably, the phosphoenolpyruvate-dependent sugar transport pathway exists only in P. putida. This pathway is closely related to phosphorylation and fructose uptake. It determines fructose availability by regulating Cra proteins and intracellular redox state.

CD BioGlyco is committed to providing high-quality and efficient services to our clients. Whether you want to build a chassis cell or develop chassis, we provide you with the best quality and most convenient solution. Please feel free to contact us.

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