Transforming Concepts into Reality with Synthetic Biology

Synthetic biology is an interdisciplinary field that brings together biology, genomics, engineering, and informatics to effectively apply systems biology and engineering thinking to the design, modification, and reconstruction of biomolecules and biological components. In recent years, synthetic biology has had a great impact on the chemical, food, energy, and medical fields. As a company specializing in glycobiology research, CD BioGlyco possesses cutting-edge synthetic biology technology and provides high-quality glycan biomolecule production services to clients all over the world.

Glycan Biomolecule Production Service at CD BioGlyco Based on Synthetic Biology

• Microbial cell factories (MCFs)
  • MCF technology is a core aspect of green biomanufacturing. By leveraging the powerful and diverse biochemical reaction networks of microorganisms, microbial cells can be transformed into MCFs capable of producing various products from low-value renewable resources by remodeling and engineering metabolic pathways. We use genome-wide reorganization approaches, novel mutagenesis methods, and genomic library co-expression/co-expression to construct MCFs at the genome-wide level and use them for the production of saccharides.
  • We develop cell factories by parsing the synthetic pathways of glycan biomolecules and reconfiguring them in microbial cells to enable the fermentative synthesis of target substances using renewable feedstocks. For example, in Yeast, we construct cell factories that utilize glycosyltransferases to synthesize carbohydrate antigens or natural glycans from scratch.
• Genetic Engineering
  • The genetic engineering technologies we offer are centered around heterologous synthesis, yield enhancement, and custom synthesis. In the production of glyco-biomolecules, we provide CRISPR-based gene editing tools to genetically modify (transposon insertion and homologous recombination) the microorganisms that produce glyco-biomolecules. In this process, the CRISPR gene editing toolkit for Pseudomonas spp. can perform mutations, deletions, insertions, and single-base editing with nearly 100% efficiency. This method can greatly accelerate the screening of yield-enhancing genes and the construction of engineered bacteria.
  • On the other hand, we employ abundant gene expression regulatory elements to aid in the production of glyco-biomolecules. In general, the production of glyco-biomolecules is closely related to the expression of enzymes; therefore, we regulate the expression of genes on a large scale by constructing enzyme expression promoter libraries.
• Cell Fermentation
  • Cell fermentation is also an effective means of producing glyco-biomolecules. We scale up the production of glyco-biomolecules by analyzing microbial cells. For example, we analyze the utilization of different low-carbon compounds by yeast to construct yeast factories with a wider range of carbon sources. Next, we enhance the production and yield of yeast cells by mixing and scaling carbon sources.
  • In addition, our research team has opened up the synthetic pathway for the synthesis of glyco-macromolecular compounds from glyco-small molecule compounds by introducing different synthetic pathways of glyco-macromolecules and regulating the endogenous glycogen synthesis and degradation pathway in yeast cells.

Fig.1 Custom synthetic biology production services. (CD BioGlyco)

Here, we provide our clients with glycan-related products that include but are not limited to the following.

HMO

HMOs are the third most abundant solid component in breast milk (after fat and lactose) and are known to regulate immunity, aid brain development, and regulate intestinal flora. CD BioGlyco offers a diverse range of production services depending on the type of HMO.

Carbohydrate Antigen

Carbohydrate antigens are now widely used in the development of vaccines for a variety of pathogenic microorganisms and cancers. CD BioGlyco offers several types of carbohydrate antigen production for clients to choose from.

Cosmetic Additive

Certain glycan molecules are also indispensable in the development of cosmetic products. CD BioGlyco provides its clients with high-quality cosmetic additive production services.

Sweetener

Sweeteners come in a wide variety of types and structures and CD BioGlyco also offers production services to clients for many types of sweeteners. The sweeteners we offer include but are not limited to, the following types.

Natural Glycan

Often synthesized by living organisms, natural glycans are biomolecules located in the cell wall, intracellular, intercellular, and extracellular in the secretory plasma, and are essential components for the activities of life. CD BioGlyco utilizes synthetic biology to provide our clients with natural glycan production services. The glycans we offer include but are not limited to, the following types.

Bioactive Ingredient

Bioactives are substances that affect life's phenomena in minute or small amounts, and saccharides are one of the more common types of bioactives. CD BioGlyco utilizes advanced synthetic biology technology to provide a wide variety of saccharide bioactives for our clients to produce. The types of bioactive substances we offer include but are not limited to, the following.

Specific Glycan Molecule

CD BioGlyco provides high-quality glycan molecule production services using world-leading biological technologies.

Applications

• The production of carbohydrate antigens can be used in the development of anti-tumor or anti-cancer vaccines.
• The glycan biomolecules are used in the development of surfactants in cosmetics and sweeteners in the food industry.
• Glycan biomolecules can regulate the immune system and can be used to investigate many biochemical reactions in the human body, such as nucleic acid synthesis, and protein and lipid metabolism.

Advantages

• We use advanced synthetic biology techniques to provide clients with custom synthesis of a wide range of glycan biomolecules.
• We have excellent biological synthesis, optimization, and scale-up technologies.
• We provide scale-up production of glycan biomolecules and analysis one-stop service.

CD BioGlyco has a professional glycan biomolecule research and development team to provide customized production services for glycan molecules using the world's leading synthetic biology technology. If you would like to know more about our production services, please feel free to contact us.

• GlycoChas™ Cells
• Chassis Development
• HMO Production Service
• Carbohydrate Antigen Production Service
• Natural Glycan Production Service
• Bioactive Ingredient Production Service
• Specific Glycan Molecule Production Service