Schizosaccharomyces pombe Engineering Service
Empowering Innovation with Schizosaccharomyces pombe Engineering
Schizosaccharomyces pombe is a simple unicellular organism that is closer to mammalian cells than brewer's yeast. S. pombe combines the use of synthetic biology-based strategies and methods to efficiently produce a wide range of high-value-added products such as biofuels, biomaterials, bulk chemicals, fine chemicals, pharmaceuticals and their intermediates, and rare natural and non-natural products.
Professional S. pombe Engineering Service at CD BioGlyco
As a company specializing in the field of biology, CD BioGlyco provides high-quality S. pombe engineering services by constructing Chassis Cell Platforms.
Promoter Development
Endogenous and heterologous promoter sequences with higher transcriptional potency, specificity, and the ability to achieve precise regulation (quantitative, timed, and targeted) can effectively increase the yield of heterologous proteins as well as other products synthesized by the S. pombe system (chassis cells). CD BioGlyco provides the development of promoters for the S. pombe system according to client needs.
Development of shorter sequences and stronger promoters by artificial systematic modifications
Enhancement of promoter activity by appropriate genetic modification and modification of existing promoters
Meanwhile, based on the excellent performance promoter, we construct more different types of S. pombe gene expression vectors to further enhance the efficiency of synthesized products in S. pombe chassis cells.
Construction of Carriers
CD BioGlyco offers several methods to construct expression vectors for S. pombe.
Screening of integrating plasmids
Screening of S. pombe centromere sequence
Screening of S. pombe autonomously replicating sequence
Gene Editing
Gene engineering is an effective means of improving the productivity of the S. pombe system. CD BioGlyco offers a variety of gene editing tools to choose from.
Cre-loxP system
Cre-loxP system mainly consists of Cre recombinase and loxP sites, which can be used to genetically modify or alter the target genes on the chromosome by utilizing the principle that Cre recombinase can specifically recognize loxP sites. Cre-loxP system is not only capable of knocking out or replacing individual genes but also efficiently completing the one-time knockout of multiple genes.
CRISPR-Cas system
The CRISPR-Cas system is an RNA-guided technology that utilizes a special nuclease such as Cas9 to perform targeted editing of target genes on S. pombe chassis cells. Compared with the traditional gene editing methods, the CRISPR-Cas system is easier to operate, and it only needs to synthesize a sgRNA to achieve specific modifications of the target gene.
In addition, we have meganucleases and transcription activator-like effector nucleases to enable gene editing of S. pombe chassis cells.
Publication Data
Technology: Gene knock-in
Journal: Microbial Cell Factories
IF: 6.4
Published: 2018
Results: In this research, the authors successfully developed a metabolic engineering of S. pombe to enhance 3-hydroxypropionic acid (3-HP) production from glucose and cellobiose via the malonyl coenzyme A pathway. To increase the cellular supply of malonyl coenzyme A and acetyl coenzyme A, the authors introduced genes encoding endogenous aldehyde dehydrogenase, acetyl coenzyme A synthase from Salmonella enterica, and endogenous pantothenate kinase into S. pombe. The authors also engineered sugar supply by demonstrating β-glucosidase (BGL) on the surface of yeast cells. Eventually, the authors succeeded in obtaining a high yield of 3-HP at a glucose concentration of 50 g/L.
Fig.1 3-HP production pathway for S. pombe. (Takayama, et al., 2018)
Applications
- Brewing industry: S. pombe converts sugar into alcohol and carbon dioxide through fermentation. Therefore, S. pombe engineering can be used in research for the production of beer, wine, and other fermented beverages.
- Biomedicine: S. pombe engineering can be used to assist in the expression and production of protein drugs, vaccines, and antibodies.
- Food industry: Yeast is used in the food industry for the fermentation of bread, dough, and other pastries. S. pombe engineering can also be used in the production of monosodium glutamate, vitamins, and other food additives.
- Biofuel: S. pombe engineering can be used in research for biofuel production. It can convert plant cellulose and other biomass into ethanol and other renewable energy sources.
Advantages
- We have a long-term commitment to research in biology and long experience in process design and selection of equipment for yeast engineering.
- We have extensive and high-volume Fungal Chassis cell culture capabilities and ensure optimal design for growth and quality.
- With world-leading synthetic biology expertise, we offer custom Chassis Cell R&D Solutions as well as the Production of many types of glycoconjugates to our clients.
CD BioGlyco has a high-quality synthetic biology platform and experienced technical staff to provide the best and most efficient project services. Clients can contact our staff directly and we will respond promptly. Please contact us for more details if you are interested in our services.
Reference
- Takayama, S.; et al. Enhancing 3-hydroxypropionic acid production in combination with sugar supply engineering by cell surface-display and metabolic engineering of Schizosaccharomyces pombe. Microbial Cell Factories. 2018, 17: 176.
For research use only. Not intended for any clinical use.
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