Bolstering Bioengineering with C. jejuni Expertise at CD BioGlyco

C. jejuni is an antigenically and phenotypically diverse bacterium with important research value in biology and medicine. As a professional biology company, CD BioGlyco utilizes the "design-construct-test-learn" strategy of synthetic biology to conduct research and construction of C. jejuni engineering and provide our clients with a wide range of C. jejuni engineering services.

"Design-construct-test-learn" strategy

• Designing biological components

  We utilize specific analysis tools for the design of biological components in C. jejuni. We design and optimize promoters, terminators, and manipulators by predicting their structures.

• Construct

  The process of construction includes DNA synthesis, large fragment assembly, and gene editing.

  We utilize a variety of methods such as microarray chips, oligonucleotide linkers mediated DNA assembly, and other methods to synthesize DNA in C. jejuni hosts and complete the seamless splicing of large fragments of DNA.

  We utilize transcription activator-like effector (TAL effector), as well as C. jejuni's endogenous clustered regularly interspaced short palindromic repeats (CRISPR)-Cas-based system to rapidly realize gene insertion, deletion, and single-base editing operations.

• Test

  We utilize a variety of high-throughput or automated screening and detection technologies to detect and analyze optimal biological components and combinations. For example, expression testing of biological components, in vitro or in vivo testing of non-natural pathways, and analysis of spatiotemporal regulation and effects on growth and metabolism after cell factory modification.

• Learn

  We analyze different levels of "gene-RNA-protein-metabolism-phenotype" and construct a knowledge map of genotype-phenotype and metabolism regulatory networks by using a systems biology approach based on histology.

Fig.1 "Design-construct-test-learn" strategy. (CD BioGlyco)

Chassis Cell Construction

We use a dual fluorescent reporter gene detection system, homologous recombination, and CRISPR-Cas system for gene knockout, insertion, site-directed mutagenesis, gene replacement, and seamless genome deletion in the C. jejuni system. After this series of operations, streamlined C. jejuni chassis cells can be efficiently prepared.

Fig.2 Chassis cell construction technologies. (CD BioGlyco)

In addition, we apply systems biology approaches such as genomics, transcriptomics, proteomics, metabolomics, and metabolic network modeling to analyze the linkage between genotypes and phenotypes of the mutant strains of C. jejuni, so that we provide abundant genomic data and genetic modification sites for the development of biocomponents and the rational design of C. jejuni, and continually improve the production efficiency of the target products.

Applications

• C. jejuni engineering helps to realize the design of artificial Chassis Cells and the construction of cell factories with different characteristics.
• C. jejuni engineering helps to discover genotypes of C. jejuni with excellent phenotypic associations such as fast growth rate and high product yield.
• C. jejuni engineering can be used to rationally design and construct genome-optimized industrial Bacterial Chassis Cells with biosafety features.

Advantages

• We combine the advantages of model and non-model cell factories, integrate multi-omics data, and design bacterial chassis cells with excellent performance.
• We keep up with the trends of the industry and continue to innovate our biology technology to provide high-quality chassis cell-based Production Services to our clients.
• From sample processing, and quality assessment to machine testing, every step of our process is strictly controlled to ensure the accuracy of results.
• We update real-time data and provide our clients with comprehensive reports in strict confidence.

CD BioGlyco has top-notch biological technology and a team of experienced experts dedicated to advancing each client's project. Our professional research team provides custom experimental solutions according to our client's needs to ensure their satisfaction. Please feel free to contact us for more details.

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