Bioactive Ingredient Production Service
Synthetic Glycobiology-Design for Bioactive Ingredient
Bioactive ingredient displays strong structural complexity. It confers important medicinal value and a variety of biological activities required for material engineering. CD BioGlyco uses multiple tools for genomics and molecular biology to make it easier to identify proteins and genes encoding for catalytic molecular biosynthesis. To increase the yield of bioactive molecules, our researchers intervene in the biosynthetic pathways and optimize the reaction conditions. With many years of experience in the field of synthetic glycobiology, CD BioGlyco provides professional GlycoChas™ Cells Generation and Chassis Development services. In addition, we provide high-quality Production services including HMO Production, Carbohydrate Antigen Production, Natural Glycan Production, and Specific Glycan Molecule Production.
Bioactive Ingredient Production Service at CD BioGlyco
CD BioGlyco helps clients produce specific bioactive molecules in small quantities or at a large scale through artificial design, biosynthesis, and gene editing. Meanwhile, our services focus on synthetic biology and metabolic engineering strategies that enable heterologous production.
- Construction
Rely on efficiently engineered strains such as yeast and E. coli.
- Biological enzyme design and modification
CD BioGlyco provides services for the design and modification of key enzymes for biosynthetic pathways. Our researchers provide design solutions for the construction and optimization of engineered strains by improving operational efficiency through efficient enzyme catalytic activity.
- Identification and characterization
CD BioGlyco provides identification and characterization services for bioactive ingredients from various biosynthetic sources. Furthermore, we provide an analysis of their key transformation pathway mechanisms.
- Custom production
Based on optimized biosynthetic routes, CD BioGlyco provides fast and accurate production of bioactive ingredients. Our high-quality production service meets your needs for use. Depending on the needs of our clients, we provide production and packaging services on a variety of scales.
In particular, our professional production team provides the following bioactive ingredient production services:
- Fructose-1,6-Bisphosphate Production
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- Anthocyanin 3-O-β-D-Glucoside Production
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- β-D-Gentiobiosyl-β-D-Glucosylcrocin Production
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- β-D-Gentiobiosylcrocin Production
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- β-Nicotinamide Mononucleotide (NMN) Production
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- Reduced Nicotinamide Mononucleotide Production
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- β-Nicotinamide Adenine Dinucleotide (Oxidized form) Production
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- β-Nicotinamide Adenine Dinucleotide Disodium Salt (Reduced form) Production
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- β-Nicotinamide Adenine Dinucleotide Phosphate Disodium Salt (Oxidized form) Production
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- β-Nicotinamide Adenine Dinucleotide Phosphate Tetrasodium Salt (Reduced form) Production
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- Nicotinamide Ribose Chloride (NR-Cl) Production
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- D-Xylulose-5-Phosphate Production
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- D-Ribulose-5-Phosphate Production
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- β-D-Glucose-6-Phosphate Production
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- α-D-Glucose-6-Phosphate Production
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- Uridine Diphosphate-Galacturonic Acid Production
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- Uridine Diphosphate-Glucuronic Acid Production
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- Kaempferol 3-O-β-D-Galactopyranoside Production
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- Glucuronic Acid Production
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- 1-O-(trans-sinapoyl)-β-D-Glucose Production
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- 1-O-(trans-cinnamoyl)-β-D-Glucose Production
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- Hydroquinone O-β-D-Glucopyranoside Production
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Publication
Technology: Mass spectra (MS), Gene knockout, DNA sequencing, liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS)
Journal: Biotechnology Journal
Published: 2024
IF: 5.726
Results: In this work, researchers used genome editing techniques to display complex N-glycosylation mutants carrying β-1,2-xylosyltransferase and α-1,3-ribosyltransferase genes. An efficient clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9 (CRISPR/cas9) program was established by genetic engineering to avoid off-target mutations. In addition, gene knockdown and expression were confirmed by DNA sequencing and MS analysis.
Fig.1 DNA sequencing results in FucT genes of the N. benthamiana line. (Kogelmann, et al., 2024)
Applications
- The deep development and research of bioactive ingredients are conducive to the discovery and development of innovative drugs.
- The metabolism and effects of bioactive ingredients in the body are usually relatively safe, so they are very useful food additives.
- Bioactive ingredients have positive effects on physiological functions, such as antioxidant, antitumor, liver protection, hypoglycemic, bacteriostatic, anti-inflammatory, antiviral, and immune regulation.
- Bioactive ingredients provide a large number of novel targets for drug screening.
Advantages of Us
- Our professional operators effectively avoid off-target and mutation failure.
- Our experienced researchers provide clients with one-stop production solutions including bioactive ingredient design, strain screening, expression optimization, small-scale testing, and mass production.
CD BioGlyco is committed to helping clients complete projects with high-quality and efficiency. Our lab has first-class technology and equipment to meet the production needs of various molecules. Please feel free to contact us.
References
- Kogelmann, B.; et al. A genome-edited N. benthamiana line for industrial-scale production of recombinant glycoproteins with targeted N-glycosylation. Biotechnol J. 2024, 19(1): e2300323.
- Xia, J.; et al. Cloning and characterization of fructose-1,6-bisphosphate aldolase from Euphausia superba. Int J Mol Sci. 2022, 23(18): 10478.
For research use only. Not intended for any clinical use.
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